Abstract

It is proposed to carry out continuous cultivation of T. pallidum in cultures of mammalian tissues. Preliminary evidence indicates that we may be observing limited multiplication. Efforts are being devoted to improvement of cultural conditions in order to demonstrate unequivocal replication of T. pallidum. Deoxyribonucleic acid (DNA) assays will be carried out to determine if simultaneous increases occur concomitantly with apparent increases in treponemal numbers. Should we be able to accomplish continuous multiplication of T. pallidum in vitro the organisms will be tested for virulence in rabbits. The isolate will also be investigated for its genetic relationship to the rabbit-passaged Nichols strain of T. pallidum from which it originated.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI023595-02
Application #
3135905
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1985-09-30
Project End
1988-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Texas College of Osteopathic Medicine
Department
Type
Schools of Osteopathy
DUNS #
City
Fort Worth
State
TX
Country
United States
Zip Code
76107