The experiments in this proposal are designed to study the significance of the association of the accumulation of unintegrated viral DNA with retroviral infections that cause cell dysfunction or death. Two model systems will be used for these studies: (1) avian leukosis virus-induced osteopetrosis; an infection in which the accumulation of unintegrated viral DNA is associated with cell dysfunction and (2) AIDS-virus infected T- cells; an infection in which the accumulation of unintegrated viral DNA occurs in cells undergoing cytopathic effects. The proposed experiments will characterize and quantitate viral and proviral DNA synthesis, the expression of viral mRNAs and the expression of viral envelope glycoproteins in cells undergoing the accumulation of unintegrated viral DNA. The proposed experiments also test the hypothesis that the accumulation of unintegrated viral DNA in different retrovirus-induced diseases is associated with a common phenomenon, the failure of the infection to establish envelope glycoprotein-mediated superinfection resistance.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI024474-03
Application #
3137498
Study Section
Virology Study Section (VR)
Project Start
1988-01-01
Project End
1990-08-31
Budget Start
1988-09-01
Budget End
1989-08-31
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of Massachusetts Medical School Worcester
Department
Type
Schools of Medicine
DUNS #
660735098
City
Worcester
State
MA
Country
United States
Zip Code
01655