The proposed research, is aimed at elucidating the cell receptor for the type D simian retrovirus (SRV-l) and at elucidating the interaction between the virus and the receptor. The proposed work will generate monoclonal anti-receptor antibodies which will be used for the isolation of the receptor. Moreover, these monoclonal antibodies will be tested for their immunogenic capacity to induce anti-idiotypic antibodies which mimic parts of the receptor, thus binding to the virus and inhibiting viral attachment to the cell receptor. The isolated cell receptor will be characterized biochemically as to molecular size and composition. The relationship between the binding of the virus or its envelope protein with the receptor, the binding of monoclonal antibodies with the receptor, and binding of synthetic peptides, representing various areas of the viral envelope protein, with the receptor will be established. Elucidation of structural features of the viral envelope protein and the cell receptor will provide a basis for analyzing interactions between these two components.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
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University of California Davis
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Torres, J V; Malley, A; Banapour, B et al. (1993) An epitope on the surface envelope glycoprotein (gp130) of simian immunodeficiency virus (SIVmac) involved in viral neutralization and T cell activation. AIDS Res Hum Retroviruses 9:423-30
Torres, J V; Anderson, D E; Malley, A et al. (1993) SIV envelope glycoprotein epitopes recognized by antibodies from infected or vaccinated rhesus macaques. J Med Primatol 22:129-37
Malley, A; Pangares, N; Mayo, S K et al. (1993) Type D SRV-2 virus-specific CD8+ and CD4- CD8- T cells that regulate virus-induced T cell proliferation in Celebes macaques. J Med Primatol 22:80-5
Malley, A; Torres, J V; Benjamini, E et al. (1992) Characterization of T cell epitopes on the envelope glycoprotein of simian retrovirus 1 and 2 (SRV-1 and SRV-2) in several mouse strains. Mol Immunol 29:999-1004
Werner, L L; Torres, J V; Leung, C Y et al. (1991) Immunobiological properties of a recombinant simian retrovirus-1 envelope protein and a neutralizing monoclonal antibody directed against it. Mol Immunol 28:819-26
Benjamini, E; Torres, J V; Werner, L L et al. (1991) Isolation and characterization of the neutralizable epitope of simian retrovirus-1 (SRV-1) and of the cell receptor for the virus. Adv Exp Med Biol 303:71-7
Torres, J V; Werner, L L; Malley, A et al. (1991) Neutralization epitope in the envelope glycoprotein of simian retrovirus-1 (SRV-1) and identification of the virus receptor. J Med Primatol 20:218-21
Malley, A; Werner, L; Benjamini, E et al. (1991) Characterization of T- and B-cell epitopes of a simian retrovirus (SRV-2) envelope protein. J Med Primatol 20:177-81
Werner, L L; Malley, A; Torres, J V et al. (1990) Synthetic peptides of envelope proteins of two different strains of simian AIDS retrovirus (SRV-1 and SRV-2) represent unique antigenic determinants for serum neutralizing antibodies. Mol Immunol 27:1103-11