The population structure of two pathogenic fungi, Histoplasma capuslatum which reproduces both sexually and asexually and Coccidioides immitis which reproduces only asexually, will be analyzed by nucleic acid variation. These population genetic data will insure the ability of PCR-mediated detection protocols to identify variable strains and isolates, and will address the recently posed, fundamental paradox that sexual fungi have a clonal population structure in nature, while some asexual fungi seem to be recombining. The PCR-mediated detection protocol will be clinically important by detecting disease rapidly, especially in immunodeficient patients. Discriminating between clonal or recombining fungal populations will be important to recognizing virulent strains or pathogenicity genes, and to understanding fungal resistance to drug therapy. The proposed study follows the lead of bacterial population biologists, and will complement ongoing studies, elsewhere, of the population biology of ascomycete pathogenic yeasts. Genomic variation will be determined at many polymorphic loci by hybridization between genomic DNA from many isolates and cloned or PCR amplified probes, or by denaturing gradient gel electrophoresis of PCR amplified regions. The presence or absence of recombination will be determined by pairwise comparison of multilocus genotypes as has been done for isozyme data, and by the new parsimony methods which borrow from phylogenetic analysis. Isolates will come from two clinical collections for each fungus, and the study will be extended to environmental isolates and international isolates. This study follows on the phylogenetic analysis of many important ascomycete fungal pathogens and their nonpathogenic relatives and both the proposed and previous studies are broadly applicable to plant pathology and food microbiology, as well as medical mycology.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
2R01AI028545-04
Application #
3143125
Study Section
Bacteriology and Mycology Subcommittee 2 (BM)
Project Start
1989-09-01
Project End
1995-08-31
Budget Start
1992-09-01
Budget End
1993-08-31
Support Year
4
Fiscal Year
1992
Total Cost
Indirect Cost
Name
University of California Berkeley
Department
Type
Schools of Earth Sciences/Natur
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704
LoBuglio, K F; Taylor, J W (1995) Phylogeny and PCR identification of the human pathogenic fungus Penicillium marneffei. J Clin Microbiol 33:85-9
Gargas, A; Taylor, J W (1995) Phylogeny of Discomycetes and early radiations of the apothecial Ascomycotina inferred from SSU rDNA sequence data. Exp Mycol 19:7-15
Gargas, A; DePriest, P T; Taylor, J W (1995) Positions of multiple insertions in SSU rDNA of lichen-forming fungi. Mol Biol Evol 12:208-18
Mitchell, T G; Freedman, E Z; White, T J et al. (1994) Unique oligonucleotide primers in PCR for identification of Cryptococcus neoformans. J Clin Microbiol 32:253-5
Burt, A; Carter, D A; White, T J et al. (1994) DNA sequencing with arbitrary primer pairs. Mol Ecol 3:523-5
Berbee, M L; Taylor, J W (1992) Convergence in ascospore discharge mechanism among pyrenomycete fungi based on 18S ribosomal RNA gene sequence. Mol Phylogenet Evol 1:59-71
Berbee, M L; Taylor, J W (1992) Two ascomycete classes based on fruiting-body characters and ribosomal DNA sequence. Mol Biol Evol 9:278-84
Bowman, B H; Taylor, J W; White, T J (1992) Molecular evolution of the fungi: human pathogens. Mol Biol Evol 9:893-904
Mitchell, T G; White, T J; Taylor, J W (1992) Comparison of 5.8S ribosomal DNA sequences among the basidiomycetous yeast genera Cystofilobasidium, Filobasidium and Filobasidiella. J Med Vet Mycol 30:207-18
Bowman, B H; Taylor, J W; Brownlee, A G et al. (1992) Molecular evolution of the fungi: relationship of the Basidiomycetes, Ascomycetes, and Chytridiomycetes. Mol Biol Evol 9:285-96

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