One of the specific aims of this application is to examine the physical characteristics of DNA:protein interactions between NFAT-1 (nuclear factor of activated T cells, a trans-activating factor that binds to a cis-acting element in the interleukin-2 [IL-2] enhancer) and cognate sites. These sites include those in the IL-2 enhancer and HIV LTR (long terminal repeat). Another goal of the investigator is to try to purify NFAT-1. The applicant also proposes to analyze the ability of the putative NFAT-1 binding site in the HIV LTR to confer inducible transcriptional activity to a linked promoter. Lastly, Dr. Edwards will try to explore the function of NFAT-1 in the life cycle of HIV, including a potential role in the initial infection of susceptible T cells and the possible involvement of NFAT-1 in the breakdown of latency. Preliminary data obtained by the applicant suggest that recognition sites for NFAT-1 have remarkable sequence divergence. Consequently, she is interested in examining the correlation between in vitro binding characteristics of NFAT-1-binding sites in IL-2 and HIV and their ability to influence the level and inducibility of transcription in vivo.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
1R01AI029856-01A1
Application #
3144796
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Project Start
1991-03-01
Project End
1996-02-29
Budget Start
1991-03-01
Budget End
1992-02-29
Support Year
1
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Genelabs Technologies, Inc.
Department
Type
DUNS #
City
Redwood City
State
CA
Country
United States
Zip Code
94063