Cryptosporidium parvum (Apicomplexa) has emerged as an important opportunistic pathogen of patients suffering from the Acquired Immunodeficiency Syndrome (AIDS). Gastrointestinal involvement may become so severe in victims of AIDS that death due to diarrheal dehydration and electrolyte imbalance may occur. Our laboratory is currently examining the basic biology of surface and endogenous proteins and glycoproteins of sporozoites of C. parvum, and propose to examine these molecules in more detail. Tbese goals will be accomplished by 1) using currently available monoclonal antibodies (Mabs) as probes to isolate and identify sporozoite proteins and glycoproteins for partial sequence analysis, 2) developing additional Mabs against sporozoite proteins and glycoproteins so that additional parasite molecules can be studied and sequenced, 3) examining whether carbohydrate moieties are a component of epitopes recognized by each Mab, and 4) examining the location of these Molecules on or within sporozoites using immunoelectron microscopy, and 5) construction of both cDNA and genomic libraries to C. parvum for use in sequencing, expressing, and studying functional domains of proteins.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI030881-03
Application #
2065952
Study Section
AIDS and Related Research Study Section 5 (ARRE)
Project Start
1992-07-01
Project End
1996-04-30
Budget Start
1994-05-01
Budget End
1996-04-30
Support Year
3
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Kansas State University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
City
Manhattan
State
KS
Country
United States
Zip Code
66506
Khramtsov, N V; Oppert, B; Montelone, B A et al. (1997) Sequencing, analysis and expression in Escherichia coli of a gene encoding a 15 kDa Cryptosporidium parvum protein. Biochem Biophys Res Commun 230:164-6
Blunt, D S; Khramtsov, N V; Upton, S J et al. (1997) Molecular karyotype analysis of Cryptosporidium parvum: evidence for eight chromosomes and a low-molecular-size molecule. Clin Diagn Lab Immunol 4:11-3
Nesterenko, M V; Woods, K M; Upton, S J (1997) Effects of manganese salts on the AIDS-related pathogen, Cryptosporidium parvum in vitro and in vivo. Biol Trace Elem Res 56:243-53
Khramtsov, N V; Blunt, D S; Montelone, B A et al. (1996) The putative acetyl-CoA synthetase gene of Cryptosporidium parvum and a new conserved protein motif in acetyl-CoA synthetases. J Parasitol 82:423-7
Blunt, D S; Montelone, B A; Upton, S J et al. (1996) Sequence of the parasitic protozoan, Cryptosporidium parvum, putative protein disulfide isomerase-encoding DNA. Gene 181:221-3
Khramtsov, N V; Tilley, M; Blunt, D S et al. (1995) Cloning and analysis of a Cryptosporidium parvum gene encoding a protein with homology to cytoplasmic form Hsp70. J Eukaryot Microbiol 42:416-22
Nesterenko, M V; Tilley, M; Upton, S J (1995) A metallo-dependent cysteine proteinase of Cryptosporidium parvum associated with the surface of sporozoites. Microbios 83:77-88
Tilley, M; Upton, S J (1994) Both CP15 and CP25 are left as trails behind gliding sporozoites of Cryptosporidium parvum (Apicomplexa). FEMS Microbiol Lett 120:275-8
Nesterenko, M V; Tilley, M; Upton, S J (1994) A simple modification of Blum's silver stain method allows for 30 minute detection of proteins in polyacrylamide gels. J Biochem Biophys Methods 28:239-42
Mitschler, R R; Welti, R; Upton, S J (1994) A comparative study of lipid compositions of Cryptosporidium parvum (Apicomplexa) and Madin-Darby bovine kidney cells. J Eukaryot Microbiol 41:8-12

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