Abstract

Pregnant HIV-1 infected women and their newborns will be subjected to a variety of virologic and immunologic evaluations. WE plan to correlate the results generated from this study of these individuals with the pregnancy outcome. This includes: a) the quantity of HIV in PBMC and plasma; b) the functional characteristics of the maternal and neonatal HIV isolates; c) the presence of CTL against autologous cells expressing gag or env of IIIb RF; d) the presence of HIV-peptide specific lymphoproliferation and/or lymphokine release; e) the titer of maternal serum neutralizing activity against autologous isolates; f) the presence of maternal antibodies which could compete with neutralizing monoclonal antibodies (anti-V3, type-specific and anti-CD4 binding site, group specific) for binding to the gp120 of JRCSF (an MN-like isolate); g) the titer of maternal ADCC (versus IIIb); h) evidence of placental inflammation and HIV infection of dissociated trophoblasts; and i) the maternal and neonatal HLA types. The correlation of assay parameters generated on the entire cohort of mother-child pairs may identify factors which increase or decrease the rate of HIV transmission. Biologic HIV-1 clones will be generated for use in assays (neutralization, ADCC, and CTL) which measure responses to autologous viral isolates. This aspect of the study will help elucidate the role of viral escape mutants in HIV transmission.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI032427-04
Application #
2067311
Study Section
AIDS and Related Research Study Section 1 (ARRA)
Project Start
1991-09-30
Project End
1996-07-31
Budget Start
1994-08-01
Budget End
1996-07-31
Support Year
4
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
New York University
Department
Pediatrics
Type
Schools of Medicine
DUNS #
004514360
City
New York
State
NY
Country
United States
Zip Code
10012

  Publications

Pollack, H; Zhan, M X; Safrit, J T et al. (1997) CD8+ T-cell-mediated suppression of HIV replication in the first year of life: association with lower viral load and favorable early survival. AIDS 11:F9-13
Pollack, H; Glasberg, H; Lee, E et al. (1997) Impaired early growth of infants perinatally infected with human immunodeficiency virus: correlation with viral load. J Pediatr 130:915-22
Cao, Y; Qin, L; Zhang, L et al. (1996) Characterization of long-term survivors of human immunodeficiency virus type 1 infection. Immunol Lett 51:7-13
Papaevangelou, V; Pollack, H; Riguad, M et al. (1996) The amount of early p24 antigenemia and not the time of first detection of virus predicts the clinical outcome of infants vertically infected with human immunodeficiency virus. J Infect Dis 173:574-8
Zhu, T; Wang, N; Carr, A et al. (1995) Evidence for coinfection by multiple strains of human immunodeficiency virus type 1 subtype B in an acute seroconvertor. J Virol 69:1324-7
Kostrikis, L G; Bagdades, E; Cao, Y et al. (1995) Genetic analysis of human immunodeficiency virus type 1 strains from patients in Cyprus: identification of a new subtype designated subtype I. J Virol 69:6122-30
Cao, Y; Qin, L; Zhang, L et al. (1995) Virologic and immunologic characterization of long-term survivors of human immunodeficiency virus type 1 infection. N Engl J Med 332:201-8
Gauduin, M C; Safrit, J T; Weir, R et al. (1995) Pre- and postexposure protection against human immunodeficiency virus type 1 infection mediated by a monoclonal antibody. J Infect Dis 171:1203-9
Huang, Y; Zhang, L; Ho, D D (1995) Biological characterization of nef in long-term survivors of human immunodeficiency virus type 1 infection. J Virol 69:8142-6
Safrit, J T; Lee, A Y; Andrews, C A et al. (1994) A region of the third variable loop of HIV-1 gp120 is recognized by HLA-B7-restricted CTLs from two acute seroconversion patients. J Immunol 153:3822-30

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