This is a second revision of a competitive renewal to test the hypothesis that the membrane skeleton is an integral structural component of the PMN microbicidal oxidase system and, hence, plays a critical role in assembly of the NADPH-dependent oxidase of human neutrophils. To this end, the proposed studies will address two specific aims: 1) identification of domains of the cytosolic oxidase component p47phox which mediate assembly of the oxidase; and 2) identification of components of the membrane skeleton associated with the assembled oxidase. It is suggested that results from these studies will provide insights concerning the structural determinants which integrate the assembly of an active oxidase with reorganization of the cytoskeleton during PMN stimulation.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI034879-10
Application #
2004004
Study Section
Special Emphasis Panel (ZRG5-BM-1 (01))
Project Start
1988-09-30
Project End
2000-11-30
Budget Start
1996-12-01
Budget End
1997-11-30
Support Year
10
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Iowa
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
041294109
City
Iowa City
State
IA
Country
United States
Zip Code
52242
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