. This proposal addresses the possible role of TNF-alpha and nitric oxide in tuberculosis reactivation. Efforts will be directed toward evaluating the significance of INF-gamma, TNF- alpha and macrophage nitric oxide synthase (iNOS), which play critical roles in host defence, in the latent and reactivation phase of infection. These will be studied in a murine model with disruption of the TNF receptor gene. Results will be analyzed by several parameters, such as histopathologic examination, immunohistochemical studies to define iNOS expression and cellular components of granulomas and reverse transcription and PCR to evaluate cytokine expression. These would be correlated with tissue bacterial load and disease progression. It is expected that these studies will yield information that could afford a better understanding of the natural history of tuberculosis and ultimately lead to the development of better therapeutic and preventive strategies.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI036990-05
Application #
6169957
Study Section
AIDS and Related Research Study Section 5 (ARRE)
Program Officer
Sizemore, Christine F
Project Start
1996-04-01
Project End
2001-03-31
Budget Start
2000-04-01
Budget End
2001-03-31
Support Year
5
Fiscal Year
2000
Total Cost
$386,727
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
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