The Plasmodium falciparum very early gametocyte stage gene, Pfg27/25, has a polymorphic locus upstreasm of the coding sequences which is surrounded by conserved sequences. The structure of the polymorphic region specifies accurate or altered blood stage specific transcription of the gene. The polymorphic structures arise by an as yet uncharacterizes DNA rearrangement. The overall objectives of this proposal are to determine the detailed structure of this locus in laboratory isolates that display different blood satge specific transcription patterns for the Pfg17/25 gene, and the extent to which these polymorphisms occur in nature. The 5' terminus of the Pfg27/25 mRNAs will also be determined to see where the transcription start sites occur with respect to the boundaries of the polymorphic domain.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI040311-02
Application #
2887295
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Program Officer
Fairfield, Alexandra
Project Start
1998-05-01
Project End
2002-04-30
Budget Start
1999-05-01
Budget End
2000-04-30
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
New York University
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10016
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Bhanot, Purnima; Frevert, Ute; Nussenzweig, Victor et al. (2003) Defective sorting of the thrombospondin-related anonymous protein (TRAP) inhibits Plasmodium infectivity. Mol Biochem Parasitol 126:263-73