Abstract

This revised proposal concerns further development of knowledge on the interaction of mycoplasmas (mollicutes) and eukaroytic cells, using techniques that measure cytadherence and intracellular invasion of three important Mycoplasma species of man (M. penetrans, M. genitalium, and M. pneumoniae) to selected target mammalian cells. As an extension of the group's earlier studies on mycoplasmal adherence and pathogenicity, the research strategy will involve application of a variety of microscopic techniques, cellular fractionation, fluorescence- labeling and cell sorting, and other biochemical, immunological, and genetic approaches to define the events following mollicute adherence and intracellular invasion. The availability of various mutant strains of the three Mycoplasma species, deficient in such selected properties as cytadherence and intracellular invasion, offers a unique avenue to clarify specific mechanisms of mycoplasmal pathogenesis, including key alterations in cell structure and function and other molecular events relevant to understanding immunologic and cellular manifestations of human mycoplasmal disease.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI041010-05
Application #
6169959
Study Section
Special Emphasis Panel (ZRG5-BM-2 (02))
Program Officer
Taylor, Christopher E,
Project Start
1996-07-01
Project End
2002-06-30
Budget Start
2000-07-01
Budget End
2002-06-30
Support Year
5
Fiscal Year
2000
Total Cost
$424,793
Indirect Cost

  Institution

Name
University of Texas Health Science Center San Antonio
Department
Microbiology/Immun/Virology
Type
Other Domestic Higher Education
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229

  Publications

Saikolappan, Sankaralingam; Sasindran, Smitha J; Yu, Hongwei D et al. (2009) The Mycoplasma genitalium MG_454 gene product resists killing by organic hydroperoxides. J Bacteriol 191:6675-82
Ueno, Priscilla M; Timenetsky, Jorge; Centonze, Victoria E et al. (2008) Interaction of Mycoplasma genitalium with host cells: evidence for nuclear localization. Microbiology 154:3033-41
Musatovova, Oxana; Herrera, Caleb; Baseman, Joel B (2006) Proximal region of the gene encoding cytadherence-related protein permits molecular typing of Mycoplasma genitalium clinical strains by PCR-restriction fragment length polymorphism. J Clin Microbiol 44:598-603
Musatovova, Oxana; Dhandayuthapani, Subramanian; Baseman, Joel B (2006) Transcriptional heat shock response in the smallest known self-replicating cell, Mycoplasma genitalium. J Bacteriol 188:2845-55
Baseman, Joel B; Cagle, Marianna; Korte, Jeffrey E et al. (2004) Diagnostic assessment of Mycoplasma genitalium in culture-positive women. J Clin Microbiol 42:203-11
Blaylock, Mark W; Musatovova, Oxana; Baseman, Janet G et al. (2004) Determination of infectious load of Mycoplasma genitalium in clinical samples of human vaginal cells. J Clin Microbiol 42:746-52
Musatovova, Oxana; Dhandayuthapani, Subramanian; Baseman, Joel B (2003) Transcriptional starts for cytadherence-related operons of Mycoplasma genitalium. FEMS Microbiol Lett 229:73-81
Alvarez, Rene A; Blaylock, Mark W; Baseman, Joel B (2003) Surface localized glyceraldehyde-3-phosphate dehydrogenase of Mycoplasma genitalium binds mucin. Mol Microbiol 48:1417-25
Dhandayuthapani, Subramanian; Rasmussen, Wanda G; Baseman, Joel B (2002) Stability of cytadherence-related proteins P140/P110 in Mycoplasma genitalium requires MG218 and unidentified factors. Arch Med Res 33:1-5
Dhandayuthapani, S; Blaylock, M W; Bebear, C M et al. (2001) Peptide methionine sulfoxide reductase (MsrA) is a virulence determinant in Mycoplasma genitalium. J Bacteriol 183:5645-50

Showing the most recent 10 out of 16 publications