The goal of this proposal is to more fully understand the role of the Gag proteins in the assembly of HIV-1 virions, with an emphasis on understanding the biochemical basis of infectivity-inactivating amino acid substitutions. In particular, the primary sequence requirements for Gag multimerization will be determined, studying both HIV-1 and also other members of the immunodeficiency virus family. The role of viral RNA in Gag multimerization will also be studied. Finally, a selection of techniques will be used to assess how mutations affecting Gag multimerization impact upon HIV-1 infectivity in vitro.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI041857-02
Application #
2673091
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Project Start
1997-09-01
Project End
2001-08-31
Budget Start
1998-09-01
Budget End
1999-08-31
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Columbia University (N.Y.)
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
167204994
City
New York
State
NY
Country
United States
Zip Code
10032
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