Abstract

This is a proposal to explore the multiple nuclear localization signals present on viral proteins found in the preintegration complex. Experiments, based on experiments from the preliminary results section, are proposed to characterize the NLSs in integrase and Vpr. In addition, many experiments will be performed to comprehensively address the possibility that multiple NLSs work in concert to mediate nuclear entry, to determine the effect of mutations in identified NLSs on replication, to compare HIV with MLV, and to identify the pathway used by the virus for nuclear entry.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI041933-02
Application #
2673117
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Project Start
1997-07-01
Project End
2001-06-30
Budget Start
1998-07-01
Budget End
1999-06-30
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Bouyac-Bertoia, M; Dvorin, J D; Fouchier, R A et al. (2001) HIV-1 infection requires a functional integrase NLS. Mol Cell 7:1025-35
O'Doherty, U; Swiggard, W J; Malim, M H (2000) Human immunodeficiency virus type 1 spinoculation enhances infection through virus binding. J Virol 74:10074-80
Palmeri, D; Malim, M H (1999) Importin beta can mediate the nuclear import of an arginine-rich nuclear localization signal in the absence of importin alpha. Mol Cell Biol 19:1218-25
Fouchier, R A; Meyer, B E; Simon, J H et al. (1998) Interaction of the human immunodeficiency virus type 1 Vpr protein with the nuclear pore complex. J Virol 72:6004-13