The goal of these studies is to use an unusual SIVmac239 variant (EvT3) to determine how useage of the CXCR4 co-receptor impacts on the rate of CD4+ T-cell decline in SIV-infected macaques. The first specific aim is to identify what co-receptors are used by EvT3 in vitro. The second is to determine the significance of co-receptor useage for the infection of CD4+ T-cell subsets by SIV. In the third specific aim, the genetic determinants in the gp120 glycoprotein that influence CXCR4 useage in vitro and CD4 depletion efficiency in vivo will be investigated.
Specific aim 4 is to determine the significance of CD4+ T-cell activation for co-receptor expression and pathogenic effects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI042508-02
Application #
2887674
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Program Officer
Plaeger, Susan F
Project Start
1998-05-01
Project End
2000-04-30
Budget Start
1999-05-01
Budget End
2000-04-30
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Oregon Regional Primate Research Center
Department
Type
DUNS #
City
Beaverton
State
OR
Country
United States
Zip Code
97006
Margolin, David H; Saunders, Erika F Helmuth; Bronfin, Benjamin et al. (2002) High frequency of virus-specific B lymphocytes in germinal centers of simian-human immunodeficiency virus-infected rhesus monkeys. J Virol 76:3965-73