The devastating impact of Super Storm Sandy on the NYU Medical center was caused by the total flooding of the lower levels of the entire Medical Center, including the Smilow Vivarium, which was under 15 of water. This caused not only the loss of electric power, air circulation, running water, etc., but also the destruction of our IVIS imaging camera and the loss of all of our mice, 30 of which were at that time being used for an experiment on the effect of the AIP on kidney infections following the IV inoculation of virulent staphylococci. We had been using bacteria tagged with a luciferase reporter to monitor the fate of the infecting bacteria by imaging in the IVIS. Although our luciferase reporters worked well for the subcutaneous infection model, we found, during a subsequent experiment that our luciferase constructs did not provide a sufficiently strong signal for the detection of bacteria in the kidney abscesses which develop following IV inoculation of staphylococci and so we had to use other parameters, such as weight loss, morbidity, and mortality to evaluate the results. Consequently, we spent a considerable amount of time developing new reporters with higher luciferase activities or with fluorescent proteins (E2- crimson and iRFP) that are excited and emit in the near infra-red. The newer luciferase constructs were being used in the experiment that was in progress at the time of the storm. Since these strains were still being tested, they were stored temporarily on plates in the cold room and in a -20 freezer. Among the consequences of the storm, described in more detail in the accompanying application for a for-cost extension of our other grant, R01-AI22159, were the loss of power for these freezers and the cold room, resulting in the loss of all these temporary cultures, and in our entire supply of AIPs, which were stored in solution in a +4 refrigerator, whose temperature reached 50C during the power outage, causing hydrolysis of the thiolactone bond essential for AIP activity.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
3R01AI042783-14S1
Application #
8669288
Study Section
Prokaryotic Cell and Molecular Biology Study Section (PCMB)
Program Officer
Huntley, Clayton C
Project Start
2014-01-31
Project End
2016-01-30
Budget Start
2014-01-31
Budget End
2016-01-30
Support Year
14
Fiscal Year
2014
Total Cost
Indirect Cost
Name
New York University
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10016
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