The long-term objective of this project is to understand the virus-host interactions that govern whether a host is susceptible or resistant to a virus. For positive-strand RNA viruses, these interactions affect virus entry, genome expression, genome replication, assembly, intercellular movement, and pathogenicity. Many of the intracellular virus-host interactions involving these viruses are highly conserved between animals, plants and eukaryotic microbes. In addition, virus-host interactions can have profound effects on host development. To identify functionally important host compatibility or defense factors, Arabidopsis thaliana is used to identify mutants with altered susceptibility to picornavirus-like plant potyviruses. The mutants provide the resource to identify factors and cellular processes that affect different stages of the infection cycle, including those that are targeted by viral counterdefensive mechanisms. The experimental plan takes advantage of the unique attributes of Arabidopsis as a genetically and genomically tractable model host to address the functions of two cellular compatibility factors that were identified in screens for potyvirus susceptibility mutants. The plan also exploits Arabidopsis and Nicotiana benthamiana to explore virus-host interactions involved in virus-mediated suppression of RNA silencing defense and RNA-controlled developmental pathways during infection. The project has two major Specific Aims : . Understand the functions of two host compatibility factors - elF(iso)4E and LSP2 - in infected and non-infected cells. These proteins are required for systemic infection by potyviruses. The interactions of these proteins with viral and cellular factors, and the roles of these proteins in infected and noninfected cells, will be determined. . Understand how virus-encoded RNA silencing suppressors work to inhibit silencing and microRNA-based developmental pathways during virus infection. Interactions between several viral silencing suppressors and cellular complexes and regulatory RNAs, and the interactions between suppressors and microRNA-controlled developmental pathways, will be examined.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI043288-09
Application #
7021401
Study Section
Experimental Virology Study Section (EVR)
Program Officer
Park, Eun-Chung
Project Start
1997-09-30
Project End
2008-02-29
Budget Start
2006-03-01
Budget End
2007-02-28
Support Year
9
Fiscal Year
2006
Total Cost
$241,807
Indirect Cost
Name
Oregon State University
Department
Other Basic Sciences
Type
Schools of Arts and Sciences
DUNS #
053599908
City
Corvallis
State
OR
Country
United States
Zip Code
97339
Campo, Sonia; Gilbert, Kerrigan B; Carrington, James C (2016) Small RNA-Based Antiviral Defense in the Phytopathogenic Fungus Colletotrichum higginsianum. PLoS Pathog 12:e1005640
Garcia-Ruiz, Hernan; Carbonell, Alberto; Hoyer, J Steen et al. (2015) Roles and programming of Arabidopsis ARGONAUTE proteins during Turnip mosaic virus infection. PLoS Pathog 11:e1004755
Carbonell, Alberto; Fahlgren, Noah; Mitchell, Skyler et al. (2015) Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric miRNA precursors. Plant J 82:1061-75
Carbonell, Alberto; Carrington, James C (2015) Antiviral roles of plant ARGONAUTES. Curr Opin Plant Biol 27:111-7
Minoia, Sofia; Carbonell, Alberto; Di Serio, Francesco et al. (2014) Specific argonautes selectively bind small RNAs derived from potato spindle tuber viroid and attenuate viroid accumulation in vivo. J Virol 88:11933-45
Carbonell, Alberto; Takeda, Atsushi; Fahlgren, Noah et al. (2014) New generation of artificial MicroRNA and synthetic trans-acting small interfering RNA vectors for efficient gene silencing in Arabidopsis. Plant Physiol 165:15-29
Gilbert, Kerrigan B; Fahlgren, Noah; Kasschau, Kristin D et al. (2014) Preparation of Multiplexed Small RNA Libraries From Plants. Bio Protoc 4:
Zhang, Xiaoming; Niu, DongDong; Carbonell, Alberto et al. (2014) ARGONAUTE PIWI domain and microRNA duplex structure regulate small RNA sorting in Arabidopsis. Nat Commun 5:5468
Fahlgren, Noah; Bollmann, Stephanie R; Kasschau, Kristin D et al. (2013) Phytophthora have distinct endogenous small RNA populations that include short interfering and microRNAs. PLoS One 8:e77181
Jeong, Dong-Hoon; Schmidt, Skye A; Rymarquis, Linda A et al. (2013) Parallel analysis of RNA ends enhances global investigation of microRNAs and target RNAs of Brachypodium distachyon. Genome Biol 14:R145

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