Abstract

This is a 3 year revised application that proposes to provide a working model using antibodies, Fab's/F(ab')2's or scFvs to block sporozoite (from P. gallinaceum) invasion of salivary glands in mosquitoes (A. aegypti). The ultimate goal is to be able to insert genes for these scFv's into mosquitoes using recombinant methods so that the mosquitoes will not be suitable hosts for sporozoite invasion and transmission. The idea is that results from these research projects will be relatively easily transferred to the P. falciparum sporozoites and their vectors, anopholine mosquitoes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
1R01AI044800-01A1
Application #
6040614
Study Section
Special Emphasis Panel (ZRG1-TMP (01))
Program Officer
Aultman, Kathryn S
Project Start
2000-02-01
Project End
2003-01-31
Budget Start
2000-02-01
Budget End
2001-01-31
Support Year
1
Fiscal Year
2000
Total Cost
$257,552
Indirect Cost

  Institution

Name
University of California Irvine
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

Jasinskiene, Nijole; Coleman, Judy; Ashikyan, Aurora et al. (2007) Genetic control of malaria parasite transmission: threshold levels for infection in an avian model system. Am J Trop Med Hyg 76:1072-8
Atkinson, Peter W; James, Anthony A (2002) Germline transformants spreading out to many insect species. Adv Genet 47:49-86
de Lara Capurro, M; Coleman, J; Beerntsen, B T et al. (2000) Virus-expressed, recombinant single-chain antibody blocks sporozoite infection of salivary glands in Plasmodium gallinaceum-infected Aedes aegypti. Am J Trop Med Hyg 62:427-33