This proposal seeks basic information on the transmission of pediculosis and the development of molecular-based diagnostic methods that will greatly improve and speed our ability to detect pyrethroid and lindane resistance in head lice and to better and more safely manage head louse infestations worldwide.
The specific aims are: 1) using toxicokinetic and toxicodynamic approaches, determine the biochemical mechanisms underlying lindane and permethrin resistance and to elucidate additional resistance pathways; 2) Using standard PCR, cloning and sequencing techniques, identify corresponding mutations in the GABA receptor subunit gene and in the sodium channel a-subunit gene that have been shown to cause target site insensitivity resistance to lindane permethrin in other insects, respectively; and using these mutations, develop monitoring methods (SSCP, minisequencing, cPASA) for resistance management; 3) Using behavioral assays, determine the importance of direct contact versus formites in transmission; 4) Design an artificial feeding system, using a suitable membrane/blood source, enabling the rearing of pediculicide susceptible and resistant """"""""reference strains"""""""" in the laboratory for toxicological investigations and for the determination of suitable replacement/alternative pediculicides.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
6R01AI045062-02
Application #
6374116
Study Section
Special Emphasis Panel (ZRG1-TMP (01))
Program Officer
Aultman, Kathryn S
Project Start
2000-09-15
Project End
2003-08-31
Budget Start
2001-09-01
Budget End
2002-08-31
Support Year
2
Fiscal Year
2001
Total Cost
$153,500
Indirect Cost
Name
University of Massachusetts Amherst
Department
Zoology
Type
Schools of Earth Sciences/Natur
DUNS #
153223151
City
Amherst
State
MA
Country
United States
Zip Code
01003
Kim, J H; Previte, D J; Yoon, K S et al. (2017) Comparison of the proliferation and excretion of Bartonella quintana between body and head lice following oral challenge. Insect Mol Biol 26:266-276
Clark, J Marshall; Yoon, Kyong Sup; Kim, Ju Hyeon et al. (2015) Utilization of the human louse genome to study insecticide resistance and innate immune response. Pestic Biochem Physiol 120:125-32
Kwon, Deok Ho; Kim, Ju Hyeon; Kim, Young Ho et al. (2014) Identification and characterization of an esterase involved in malathion resistance in the head louse Pediculus humanus capitis. Pestic Biochem Physiol 112:13-8
Yoon, Kyong Sup; Previte, Domenic J; Hodgdon, Hilliary E et al. (2014) Knockdown resistance allele frequencies in North American head louse (Anoplura: Pediculidae) populations. J Med Entomol 51:450-7
Olds, Brett P; Coates, B S; Steele, L D et al. (2012) Comparison of the transcriptional profiles of head and body lice. Insect Mol Biol 21:257-68
Strycharz, Joseph P; Lao, Alice R; Alves, Anna-Maria et al. (2012) Ovicidal response of NYDA formulations on the human head louse (Anoplura: Pediculidae) using a hair tuft bioassay. J Med Entomol 49:336-42
Strycharz, Joseph P; Berge, Noah M; Alves, Anna-Maria et al. (2011) Ivermectin acts as a posteclosion nymphicide by reducing blood feeding of human head lice (Anoplura: Pediculidae) that hatched from treated eggs. J Med Entomol 48:1174-82
Kim, Ju Hyeon; Min, Jee Sun; Kang, Jae Soon et al. (2011) Comparison of the humoral and cellular immune responses between body and head lice following bacterial challenge. Insect Biochem Mol Biol 41:332-9
Yoon, K S; Strycharz, J P; Baek, J H et al. (2011) Brief exposures of human body lice to sublethal amounts of ivermectin over-transcribes detoxification genes involved in tolerance. Insect Mol Biol 20:687-99
Hodgdon, Hilliary E; Yoon, Kyong Sup; Previte, Domenic J et al. (2010) Determination of knockdown resistance allele frequencies in global human head louse populations using the serial invasive signal amplification reaction. Pest Manag Sci 66:1031-40

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