Abstract

The proposed research will focus on G-protein coupled receptors GPCR in Aedes aegypti. The applicant utilized sequence homology to obtain two PCR products putatively corresponding to two hormone GPCR. The applicant proposes to test the hypothesis that the cDNA probes will identify receptors that are expressed in the Malpighian tubules of A. Aegypti. To test the hypothesis, the applicant proposes to clone the receptor gene, localize the tissue expression by in situ hybridization and immunohistochemistry and express the protein in transformed cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI046447-03
Application #
6488745
Study Section
Special Emphasis Panel (ZRG1-TMP (01))
Program Officer
Aultman, Kathryn S
Project Start
2000-01-15
Project End
2004-12-31
Budget Start
2002-01-01
Budget End
2004-12-31
Support Year
3
Fiscal Year
2002
Total Cost
$141,260
Indirect Cost

  Institution

Name
Texas A&M University
Department
Zoology
Type
Schools of Earth Sciences/Natur
DUNS #
047006379
City
College Station
State
TX
Country
United States
Zip Code
77845

  Publications

Lu, Hsiao-Ling; Kersch, Cymon; Pietrantonio, Patricia V (2011) The kinin receptor is expressed in the Malpighian tubule stellate cells in the mosquito Aedes aegypti (L.): a new model needed to explain ion transport? Insect Biochem Mol Biol 41:135-40
Jagge, C L; Pietrantonio, P V (2008) Diuretic hormone 44 receptor in Malpighian tubules of the mosquito Aedes aegypti: evidence for transcriptional regulation paralleling urination. Insect Mol Biol 17:413-26
Taneja-Bageshwar, Suparna; Strey, Allison; Zubrzak, Pawel et al. (2006) Comparative structure-activity analysis of insect kinin core analogs on recombinant kinin receptors from Southern cattle tick Boophilus microplus (Acari: Ixodidae) and mosquito Aedes aegypti (Diptera: Culicidae). Arch Insect Biochem Physiol 62:128-40
Pietrantonio, P V; Jagge, C; Taneja-Bageshwar, S et al. (2005) The mosquito Aedes aegypti (L.) leucokinin receptor is a multiligand receptor for the three Aedes kinins. Insect Mol Biol 14:55-67
Duchesne, Laurence; Hubert, Jean-Francois; Verbavatz, Jean-Marc et al. (2003) Mosquito (Aedes aegypti ) aquaporin, present in tracheolar cells, transports water, not glycerol, and forms orthogonal arrays in Xenopus oocyte membranes. Eur J Biochem 270:422-9
Lee, D W; Pietrantonio, P V (2003) In vitro expression and pharmacology of the 5-HT7-like receptor present in the mosquito Aedes aegypti tracheolar cells and hindgut-associated nerves. Insect Mol Biol 12:561-9
Pietrantonio, P V; Jagge, C; McDowell, C (2001) Cloning and expression analysis of a 5HT7-like serotonin receptor cDNA from mosquito Aedes aegypti female excretory and respiratory systems. Insect Mol Biol 10:357-69