The proposed research will focus on G-protein coupled receptors GPCR in Aedes aegypti. The applicant utilized sequence homology to obtain two PCR products putatively corresponding to two hormone GPCR. The applicant proposes to test the hypothesis that the cDNA probes will identify receptors that are expressed in the Malpighian tubules of A. Aegypti. To test the hypothesis, the applicant proposes to clone the receptor gene, localize the tissue expression by in situ hybridization and immunohistochemistry and express the protein in transformed cells.
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|Duchesne, Laurence; Hubert, Jean-Francois; Verbavatz, Jean-Marc et al. (2003) Mosquito (Aedes aegypti ) aquaporin, present in tracheolar cells, transports water, not glycerol, and forms orthogonal arrays in Xenopus oocyte membranes. Eur J Biochem 270:422-9|
|Lee, D W; Pietrantonio, P V (2003) In vitro expression and pharmacology of the 5-HT7-like receptor present in the mosquito Aedes aegypti tracheolar cells and hindgut-associated nerves. Insect Mol Biol 12:561-9|
|Pietrantonio, P V; Jagge, C; McDowell, C (2001) Cloning and expression analysis of a 5HT7-like serotonin receptor cDNA from mosquito Aedes aegypti female excretory and respiratory systems. Insect Mol Biol 10:357-69|