An immunogenic 24 kDa calcium binding protein is found in Trypanosoma cruzi and is termed flagellar calcium binding protein (FCaBP). FcaBP is modified by the addition of myristate and palmitate to its amino terminus. These modifications mediate its localization to the flagellar plasma membrane. Calcium binding is also required for localization. Thus, FcaBP is similar to recoverin a calcium-myristoyl switch protein of the mammalian retina. FcaBP binds calcium through the third and fourth EF hand domains in distinction from recoverin which is modified only by myristate and binds calcium through the second and third EF hand domains. FcaBP is a novel calcium-acyl switch protein. We will extend our studies of FcaBP in a five-year research effort, the Specific Aims of which are: (i) determination of the mechanism for the flagellar membrane localization of FcaBP, (ii) identification of FCaBP partner proteins, (iii) elucidation of the calcium-myristoly/palmitoyl switch mechanism and (iv) characterization of Typanosoma brucei calcium acyl switch proteins.
| Emmer, Brian T; Nakayasu, Ernesto S; Souther, Christina et al. (2011) Global analysis of protein palmitoylation in African trypanosomes. Eukaryot Cell 10:455-63 |
| Maric, Danijela; McGwire, Bradford S; Buchanan, Kathryn T et al. (2011) Molecular determinants of ciliary membrane localization of Trypanosoma cruzi flagellar calcium-binding protein. J Biol Chem 286:33109-17 |
| Maric, Danijela; Epting, Conrad L; Engman, David M (2010) Composition and sensory function of the trypanosome flagellar membrane. Curr Opin Microbiol 13:466-72 |
| Emmer, Brian T; Daniels, Melvin D; Taylor, Joann M et al. (2010) Calflagin inhibition prolongs host survival and suppresses parasitemia in Trypanosoma brucei infection. Eukaryot Cell 9:934-42 |
| Emmer, Brian T; Maric, Danijela; Engman, David M (2010) Molecular mechanisms of protein and lipid targeting to ciliary membranes. J Cell Sci 123:529-36 |
| Emmer, Brian T; Souther, Christina; Toriello, Krista M et al. (2009) Identification of a palmitoyl acyltransferase required for protein sorting to the flagellar membrane. J Cell Sci 122:867-74 |
| Tyler, Kevin M; Fridberg, Alina; Toriello, Krista M et al. (2009) Flagellar membrane localization via association with lipid rafts. J Cell Sci 122:859-66 |
| Fridberg, Alina; Olson, Cheryl L; Nakayasu, Ernesto S et al. (2008) Sphingolipid synthesis is necessary for kinetoplast segregation and cytokinesis in Trypanosoma brucei. J Cell Sci 121:522-35 |
| Wingard, Jennifer N; Ladner, Jane; Vanarotti, Murugendra et al. (2008) Structural insights into membrane targeting by the flagellar calcium-binding protein (FCaBP), a myristoylated and palmitoylated calcium sensor in Trypanosoma cruzi. J Biol Chem 283:23388-96 |
| Fridberg, Alina; Buchanan, Kathryn T; Engman, David M (2007) Flagellar membrane trafficking in kinetoplastids. Parasitol Res 100:205-12 |
Showing the most recent 10 out of 12 publications
