Our objective is to study the relationship of early-life cat and dog exposure to potential persistent effects on T-cell cytokine profiles, biologic markers of atopy, and clinical atopy among a well-characterized birth cohort (n=835), the Detroit area Childhood Allergy Study (CAS), as they reach 18 years of age. Extensive early-life environmental exposure data have been collected on this cohort. Outcomes will include total IgE, allergen-specific IgE and IgG4 levels, intracellular IL-4, IL-10 and interferon (IFN)gamma in mitogen stimulated T-cells and current or history of allergic rhinitis and asthma. Hypotheses: Compared to subjects with low levels of exposure, those with high-level exposure to dogs and cats in the first year of life will exhibit: i) a persistently altered immune response at age 17, typified by a low IL-4/IFN-gamma ratio and elevated IL- 10 secretion in response to T-cell mitogenic stimulation; ii) reduced levels of allergenspecific IgE and increased allergen-specific IgG4 to common aeroallergens; and, iii) a persistently reduced risk for allergic rhinitis and asthma.
The specific aims i nclude analyzing the number of indoor pets during the first year of life, adjusting for well-established confounders and effect modifiers, for persistent effects on: i) T-cell intracellular cytokine levels of IL-4, IFN-gamma and IL-10; ii) serum levels of allergen-specific IgE (cat, dog, dust mite, ragweed, grass and alternaria) and allergen-specific IgG4 (cat, dog, grass and dust mite); iii) current and lifetime history of self-reported and doctor diagnosed allergic rhinitis and asthma. We will also iv) study the relationship between these outcomes and v) evaluate the role of biomarkers and history of atopy in each parent in the above analyses. Methods: The CAS cohort will be followed-up by questionnaire and a home visit at age 18 years. Stimulated T cell cytokine analysis, total IgE and serum allergen-specific IgE and IgG4 levels will be determined and cohort subjects and parents will answer a questionnaire regarding subject and parental history of symptoms and diagnosis of allergic rhinitis and asthma and post early life pet exposure. Blood specimens, DNA and mRNA from parents and subjects will be placed in a biorepository for future molecular epidemiology studies.
| Sitarik, Alexandra Rene; Kasmikha, Nena Sabri; Kim, Haejin et al. (2018) Breast-feeding and delivery mode modify the association between maternal atopy and childhood allergic outcomes. J Allergy Clin Immunol 142:2002-2004.e2 |
| Havstad, Suzanne; Sitarik, Alexandra R; Johnson, Christine Cole et al. (2017) Allergic sensitization in American children of Middle Eastern ethnicity at age 2. Ann Allergy Asthma Immunol 119:464-466 |
| Wegienka, G; Havstad, S; Zoratti, E M et al. (2015) Combined effects of prenatal medication use and delivery type are associated with eczema at age 2 years. Clin Exp Allergy 45:660-8 |
| Wegienka, Ganesa; Havstad, Suzanne; Zoratti, Edward M et al. (2015) Association between vitamin D levels and allergy-related outcomes vary by race and other factors. J Allergy Clin Immunol 136:1309-14.e1-4 |
| Veras, Laura; Wegienka, Ganesa; Havstad, Suzanne et al. (2014) Comparison of indoor allergen levels in urban and suburban Detroit. J Allergy Clin Immunol 134:1189-90 |
| Bassirpour, Gillian; Zoratti, Edward (2014) Cockroach allergy and allergen-specific immunotherapy in asthma: potential and pitfalls. Curr Opin Allergy Clin Immunol 14:535-41 |
| Zoratti, Edward; Havstad, Suzanne; Wegienka, Ganesa et al. (2014) Differentiating asthma phenotypes in young adults through polyclonal cytokine profiles. Ann Allergy Asthma Immunol 113:25-30 |
| Ezell, Jerel M; Wegienka, Ganesa; Havstad, Suzanne et al. (2013) A cross-sectional analysis of pet-specific immunoglobulin E sensitization and allergic symptomatology and household pet keeping in a birth cohort population. Allergy Asthma Proc 34:504-10 |
| Burnett, Mary; Wegienka, Ganesa; Havstad, Suzanne et al. (2013) Relationship of dog- and cat-specific IgE and IgG4 levels to allergic symptoms on pet exposure. J Allergy Clin Immunol Pract 1:350-3 |
| Rajendra, Chathruckan; Zoratti, Edward; Havstad, Suzanne et al. (2012) Relationships between total and allergen-specific serum IgE concentrations and lung function in young adults. Ann Allergy Asthma Immunol 108:429-34 |
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