The number of available genome sequences from the bacterial family Enterobacteriaceae is reaching a threshold where comparative genomics can drive hypotheses and experiments. In this project we have selected genomes for sequencing based on their pathogenicity and their taxonomic position. These sequences will help us understand these and other related pathogens by defining their differences and similarities in gene content. (1) The genome sequences of S. enterica serovar Paratyphi A (SPA), already sampled to 97 percent coverage, will be completed and annotated. SPA is the second most prevalent cause of typhoid and, like S. enterica serovar Typhi (STY), is restricted to humans. Typhi is undergoing genome degradation, perhaps associated with its recent adaptation to a narrow host range; we will determine if Paratyphi A is undergoing similar degradation. Klebsiella pneumoniae is a major opportunistic pathogen. We have sequenced this genome to 8-fold coverage; it will be closed, finished and annotated. (2) Cost-effective four-fold sampling (97 percent coverage) will be performed for four genomes: a biotype of S. enterica Paratyphi B (SPB), which is the third most prevalent cause of typhoid and is host-adapted to man; S. enterica Arizonae (SAR), the most distantly related S. enterica that regularly causes disease in humans; Citrobacter koseri (CKO) and Enterobacter cloacae (ECL) both of which are opportunistic pathogens representing the unsequenced genera within or adjacent to the E. coli/Salmonella/Klebsiella clade. Web-based analysis tools that take into account the incomplete nature of the samples will be used to present these data in comparison to other related genomes. Finally, (3) we have amplified and arrayed the complete open reading frames of nearly every CDS in S. enterica subspecies 1, serovar Typhimurium LT2. This resource will be supplemented with new putative CDSs, not found in STM, as these sequences become available from STY, SPA, SPB, and other serovars of S. enterica. Thus, we will develop an array that can be used in a wide variety of Salmonella, both sequenced and unsequenced, for analysis of expression and of genome content.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI052237-02
Application #
6649304
Study Section
Genome Study Section (GNM)
Program Officer
Schmitt, Clare K
Project Start
2002-09-01
Project End
2005-08-31
Budget Start
2003-09-01
Budget End
2004-08-31
Support Year
2
Fiscal Year
2003
Total Cost
$661,600
Indirect Cost
Name
Washington University
Department
Genetics
Type
Schools of Medicine
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Gupta, Sushim K; McMillan, Elizabeth A; Jackson, Charlene R et al. (2016) Draft Genome Sequence of Salmonella enterica subsp. diarizonae Serovar 61:k:1,5,(7) Strain CRJJGF_00165 (Phylum Gammaproteobacteria). Genome Announc 4:
Elhadad, Dana; Desai, Prerak; Grassl, Guntram A et al. (2016) Differences in Host Cell Invasion and Salmonella Pathogenicity Island 1 Expression between Salmonella enterica Serovar Paratyphi A and Nontyphoidal S. Typhimurium. Infect Immun 84:1150-1165
Cox, Clayton E; Wright, Anita C; McClelland, Michael et al. (2016) Influence of Salmonella enterica Serovar Typhimurium ssrB on Colonization of Eastern Oysters (Crassostrea virginica) as Revealed by a Promoter Probe Screen. Appl Environ Microbiol 82:328-39
Silva-Valenzuela, Cecilia A; Desai, Prerak T; Molina-Quiroz, Roberto C et al. (2016) Solid tumors provide niche-specific conditions that lead to preferential growth of Salmonella. Oncotarget 7:35169-80
Gupta, Sushim K; McMillan, Elizabeth A; Jackson, Charlene R et al. (2016) Draft Genome Sequence of Salmonella enterica subsp. enterica Serovar Putten Strain CRJJGF_00159 (Phylum Gammaproteobacteria). Genome Announc 4:
Gupta, Sushim K; McMillan, Elizabeth A; Jackson, Charlene R et al. (2016) Draft Genome Sequence of Salmonella enterica subsp. enterica Serovar Lille Strain CRJJGF_000101 (Phylum Gammaproteobacteria). Genome Announc 4:
Gupta, Sushim K; McMillan, Elizabeth A; Jackson, Charlene R et al. (2016) Draft Genome Sequence of Salmonella enterica subsp. enterica Serovar Orion Strain CRJJGF_00093 (Phylum Gammaproteobacteria). Genome Announc 4:
Gupta, Sushim K; McMillan, Elizabeth A; Jackson, Charlene R et al. (2016) Draft Genome Sequence of Salmonella enterica subsp. enterica Serovar Bardo Strain CRJJGF_00099 (Phylum Gammaproteobacteria). Genome Announc 4:
Gupta, Sushim K; McMillan, Elizabeth A; Jackson, Charlene R et al. (2016) Draft Genome Sequence of Salmonella enterica subsp. enterica Serovar Blockley Strain CRJJGF_00147 (Phylum Gammaproteobacteria). Genome Announc 4:
Yang, Hee-Jeong; Bogomolnaya, Lydia M; Elfenbein, Johanna R et al. (2016) Novel Two-Step Hierarchical Screening of Mutant Pools Reveals Mutants under Selection in Chicks. Infect Immun 84:1226-1238

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