(Revised Abstract) The long range objective of the proposed study is to gain insight into the pathogenic mechanisms of Bartonella, an opportunistic pathogen of AIDS patients. B. henselae and B. quintana are fastidious, gram-negative bacteria that cause bacillary angiomatosis (BA), a vascular proliferative lesion affecting HIV-infected patients. Relapsing and/or persistent bloodstream infection is a frequent manifestation of B. quintana infection that occurs in patients at all stages of HIV infection and can last for months in humans. Causing debilitating and even fatal sequelae. We recently identified a protein of Bartonella that has a number of characteristics in common with other virulence determinants of bacterial pathogens that are able to successfully and persistently infect the human host. This protein appears to be involved in Bartonella pathogenesis in humans. The immediate objective of this proposal is to study the mechanisms of Bartonella pathogenesis by elucidating the virulence properties of the B. quintana protein, including characterization of the interactions with the host in vitro and in vivo, and the clinical and molecular correlation of expression of this protein in isolates from AIDS patients. The ultimate goal of this project is to identify the contribution of this protein to Bartonella-mediated pathogenesis in HIV-infected patients at the bacterial and host molecular and cellular levels.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
1R01AI052813-01A1
Application #
6590932
Study Section
AIDS and Related Research 8 (AARR)
Program Officer
Lambros, Chris
Project Start
2002-12-15
Project End
2007-11-30
Budget Start
2002-12-15
Budget End
2003-11-30
Support Year
1
Fiscal Year
2003
Total Cost
$485,610
Indirect Cost
Name
University of California San Francisco
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
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Lee, Sulggi A; Plett, Sara K; Luetkemeyer, Anne F et al. (2015) Bartonella quintana Aortitis in a Man with AIDS, Diagnosed by Needle Biopsy and 16S rRNA Gene Amplification. J Clin Microbiol 53:2773-6
Previte, D; Olds, B P; Yoon, K et al. (2014) Differential gene expression in laboratory strains of human head and body lice when challenged with Bartonella quintana, a pathogenic bacterium. Insect Mol Biol 23:244-54
Abromaitis, Stephanie; Koehler, Jane E (2013) The Bartonella quintana extracytoplasmic function sigma factor RpoE has a role in bacterial adaptation to the arthropod vector environment. J Bacteriol 195:2662-74
Abromaitis, Stephanie; Nelson, Christopher S; Previte, Domenic et al. (2013) Bartonella quintana deploys host and vector temperature-specific transcriptomes. PLoS One 8:e58773
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Vigil, Adam; Ortega, Rocio; Jain, Aarti et al. (2010) Identification of the feline humoral immune response to Bartonella henselae infection by protein microarray. PLoS One 5:e11447
Henn, Jennifer B; Gabriel, Mourad W; Kasten, Rickie W et al. (2009) Infective endocarditis in a dog and the phylogenetic relationship of the associated ""Bartonella rochalimae"" strain with isolates from dogs, gray foxes, and a human. J Clin Microbiol 47:787-90
Bouchouicha, Rim; Durand, Benoit; Monteil, Martine et al. (2009) Molecular epidemiology of feline and human Bartonella henselae isolates. Emerg Infect Dis 15:813-6
Chomel, Bruno B; Henn, Jennifer B; Kasten, Rickie W et al. (2009) Dogs are more permissive than cats or guinea pigs to experimental infection with a human isolate of Bartonella rochalimae. Vet Res 40:27

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