: Babesia bovis is a protozoal intraerythrocytic parasite that establishes persistent infections of extremely long duration. Although recognition of parasite antigens on the infected red blood cell (IRBC) membrane surface by antibodies may provide protection from disease, the host cannot develop immunity to infection. At least two mechanisms are used by this parasite to establish persistent infection: [1] cytoadhesion of IRBCs to the micro-vascular endothelium prevents mature parasite stages from circulating, thus avoiding the spleen; and [2] antigenic variation of the parasite components on the IRBC surface """"""""protects"""""""" the cytoadhesive ligand from immune recognition, allowing variant parasites to persist. Strong circumstantial evidence implicates the VESA1 antigen in cytoadhesion, identifying the VESA1 as a bi-functional virulence factor of immune evasion. Previously, we identified the B. bovis ves multigene family encoding the VESA1a polypeptide, a major participant in antigenic variation on the IRBC surface, and found evidence this occurs through segmental gene conversion. Recently, we have discovered this gene family to be consistently organized as palindromic (head-to-head) gene pairs, without obvious differences in the upstream regulatory sequences of transcribed lnd non-transcribed copies. However, this organization is likely significant to the regulation of expression of his polypeptide. We therefore propose to determine the general mechanisms responsible for regulating expression of the variant antigens, by completion of the following aims:
Aim 1 : To detect and discriminate between allelic exclusion and post-transcriptional mechanisms in the control of VESA1a expression.
Aim 2 : To determine whether higher-order in vivo structure in the 5' end of the vesla gene differs between sequence donor and actively expressed (sequence recipient) ves1a genes, and to determine the identity of the """"""""pseudogenes"""""""" apposing many ves1a genes.
Aim 3 : To determine the chromosomal location and define the full structure of a known ves1a site of transcription. The outcomes of this project will define the gross mechanisms used by this parasite to survive, and will direct future work targeting the enzymatic machinery of this and other microbes using similar strategies as a means to limit parasite survival.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI055864-04
Application #
7185058
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Program Officer
Mcgugan, Glen C
Project Start
2004-03-01
Project End
2009-02-28
Budget Start
2007-03-01
Budget End
2008-02-29
Support Year
4
Fiscal Year
2007
Total Cost
$310,411
Indirect Cost
Name
University of Florida
Department
Pathology
Type
Schools of Veterinary Medicine
DUNS #
969663814
City
Gainesville
State
FL
Country
United States
Zip Code
32611
Jackson, Andrew P; Otto, Thomas D; Darby, Alistair et al. (2014) The evolutionary dynamics of variant antigen genes in Babesia reveal a history of genomic innovation underlying host-parasite interaction. Nucleic Acids Res 42:7113-31
Huang, Yingling; Xiao, Yu-Ping; Allred, David R (2013) Unusual chromatin structure associated with monoparalogous transcription of the Babesia bovis ves multigene family. Int J Parasitol 43:163-72
Wang, Xinyi; Xiao, Yu-Ping; Bouchut, Anne et al. (2012) Characterization of the unusual bidirectional ves promoters driving VESA1 expression and associated with antigenic variation in Babesia bovis. Eukaryot Cell 11:260-9
Xiao, Yu-Ping; Al-Khedery, Basima; Allred, David R (2010) The Babesia bovis VESA1 virulence factor subunit 1b is encoded by the 1beta branch of the ves multigene family. Mol Biochem Parasitol 171:81-8
Zupa?ska, Agata K; Drummond, Paul B; Swetnam, Daniele M et al. (2009) Universal primers suitable to assess population dynamics reveal apparent mutually exclusive transcription of the Babesia bovis ves1alpha gene. Mol Biochem Parasitol 166:47-53
Allred, David R; Barbet, Anthony F; Barry, J David et al. (2009) varDB: common ground for a shifting landscape. Trends Parasitol 25:249-52
Allred, David R; Al-Khedery, Basima (2006) Antigenic variation as an exploitable weakness of babesial parasites. Vet Parasitol 138:50-60