Gamma interferon (IFN_() is an essential cytokine for mediation of functions that are obligatory for good health, particularly that mediated through or maintained through CD4 T- helper 1 (Thl) cells. Further, IFN_, possesses direct antiviral and anticellular (antitumor) activity. Thus, the development of IFN1, mimetics has important implications for immune- therapy in infectious diseases and cancer.
The Specific Aims listed below directly test both the rational development of such mimetics as well as their use in the mediation of IFN_, activity. 1. Synthesis of IFN1, peptide mimetics based on sequence and structural similarity to biologically active IFNI, C-terminal peptides. 2. Compare several methods for delivery of IFN_, peptides intracellularly, including chemical modification and use of molecular expression systems. 3. Determine signaling function of IFN_, peptide mimetics in terms of JAK/STAT activation, upregulation of tumor suppressor gene p21 wAF/cIP, and down regulation of neu/HER-2 protooncogene in cancer cells. 4. Determine cellular function of IFN_, peptide mimetics in terms of antiviral activity, anticellular (antitumor) activity, and upregulation of MHC class II molecules. 5. Determine biological activity of IFN_, mimetic peptides in mouse models of virus infection and cancer.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
3R01AI056152-05A2S1
Application #
7638699
Study Section
Immunity and Host Defense Study Section (IHD)
Program Officer
Leitner, Wolfgang W
Project Start
2008-06-01
Project End
2010-02-28
Budget Start
2008-06-01
Budget End
2009-02-28
Support Year
5
Fiscal Year
2008
Total Cost
$100,758
Indirect Cost
Name
University of Florida
Department
Microbiology/Immun/Virology
Type
Schools of Earth Sciences/Natur
DUNS #
969663814
City
Gainesville
State
FL
Country
United States
Zip Code
32611
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