Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI056231-02
Application #
6807045
Study Section
Metallobiochemistry Study Section (BMT)
Program Officer
Hall, Robert H
Project Start
2003-09-30
Project End
2008-01-31
Budget Start
2004-02-01
Budget End
2005-01-31
Support Year
2
Fiscal Year
2004
Total Cost
$187,500
Indirect Cost
Name
Medical College of Wisconsin
Department
Biophysics
Type
Schools of Medicine
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
Limphong, Pattraranee; McKinney, Ross M; Adams, Nicole E et al. (2010) The metal ion requirements of Arabidopsis thaliana Glx2-2 for catalytic activity. J Biol Inorg Chem 15:249-58
Limphong, Pattraranee; Adams, Nicole E; Rouhier, Matthew F et al. (2010) Converting GLX2-1 into an active glyoxalase II. Biochemistry 49:8228-36
Hawk, Megan J; Breece, Robert M; Hajdin, Christine E et al. (2009) Differential binding of Co(II) and Zn(II) to metallo-beta-lactamase Bla2 from Bacillus anthracis. J Am Chem Soc 131:10753-62
Limphong, Pattraranee; Crowder, Michael W; Bennett, Brian et al. (2009) Arabidopsis thaliana GLX2-1 contains a dinuclear metal binding site, but is not a glyoxalase 2. Biochem J 417:323-30
Hu, Zhenxin; Spadafora, Lauren J; Hajdin, Christine E et al. (2009) Structure and mechanism of copper- and nickel-substituted analogues of metallo-beta-lactamase L1. Biochemistry 48:2981-9
Mitra, Sanghamitra; Sheppard, George; Wang, Jieyi et al. (2009) Analyzing the binding of Co(II)-specific inhibitors to the methionyl aminopeptidases from Escherichia coli and Pyrococcus furiosus. J Biol Inorg Chem 14:573-85
Breece, Robert M; Hu, Zhenxin; Bennett, Brian et al. (2009) Motion of the zinc ions in catalysis by a dizinc metallo-beta-lactamase. J Am Chem Soc 131:11642-3
Mitra, Sanghamitra; Bennett, Brian; Holz, Richard C (2009) Mutation of H63 and its catalytic affect on the methionine aminopeptidase from Escherichia coli. Biochim Biophys Acta 1794:137-43
Hartley, Mariam; Yong, Wei; Bennett, Brian (2009) Heterologous expression and purification of Vibrio proteolyticus (Aeromonas proteolytica) aminopeptidase: a rapid protocol. Protein Expr Purif 66:91-101
Limphong, Pattraranee; McKinney, Ross M; Adams, Nicole E et al. (2009) Human glyoxalase II contains an Fe(II)Zn(II) center but is active as a mononuclear Zn(II) enzyme. Biochemistry 48:5426-34

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