Infections caused by Staphylococcus aureus span a wide clinical spectrum, ranging from asymptomatic nasal carriage to endocarditis, bone and joint infections and lethal shock. Increasing rates of S. aureus infection and the emergence of community-acquired strains drive the need for an increased understanding of the virulence determinants of this emerging pathogen and evaluating the role they play in outcome of patients with S. aureus bacteremia. Evaluating the role of these virulence determinants in humans was limited by the absence of a large, well-characterized collection of bloodstream S. aureus isolates. Such a clinical resource was developed by Vance Fowler (Co-Investigator), when he created one of the world

Public Health Relevance

Genetic factors in Staphylococcus aureus play a critical role in causing and determining the severity of infection and disease. This study proposes to identify these variations in a large collection of clinically isolated, disease-causing Staphylococci by examining genes known to be involved and identifying new genes that may be involved in infection. Knowledge acquired from this study could potentially be used to develop novel and possibly preventive therapies to eradicate complicated staphylococcal infections.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Project (R01)
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Bacterial Pathogenesis Study Section (BACP)
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Huntley, Clayton C
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University of Rochester
Internal Medicine/Medicine
Schools of Dentistry
United States
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Canfield, Gregory S; Schwingel, Johanna M; Foley, Matthew H et al. (2013) Evolution in fast forward: a potential role for mutators in accelerating Staphylococcus aureus pathoadaptation. J Bacteriol 195:615-28
Nair, Dhanalakshmi; Memmi, Guido; Hernandez, David et al. (2011) Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain. J Bacteriol 193:2332-5
Gill, Steven R; McIntyre, Lauren M; Nelson, Charlotte L et al. (2011) Potential associations between severity of infection and the presence of virulence-associated genes in clinical strains of Staphylococcus aureus. PLoS One 6:e18673
Gerrish, Robert S; Gill, Ann L; Fowler, Vance G et al. (2010) Development of pooled suppression subtractive hybridization to analyze the pangenome of Staphylococcus aureus. J Microbiol Methods 81:56-60
Gerrish, Robert S; Gill, Steven R (2010) Tailed pooled suppression subtractive hybridization (PSSH) adaptors do not alter efficiency. Antonie Van Leeuwenhoek 98:573-9
Bae, In-Gyu; Federspiel, Jerome J; Miró, José M et al. (2009) Heterogeneous vancomycin-intermediate susceptibility phenotype in bloodstream methicillin-resistant Staphylococcus aureus isolates from an international cohort of patients with infective endocarditis: prevalence, genotype, and clinical significance. J Infect Dis 200:1355-66
Xiong, Yan Q; Fowler, Vance G; Yeaman, Michael R et al. (2009) Phenotypic and genotypic characteristics of persistent methicillin-resistant Staphylococcus aureus bacteremia in vitro and in an experimental endocarditis model. J Infect Dis 199:201-8
Stryjewski, Martin E; Szczech, Lynda A; Benjamin Jr, Daniel K et al. (2007) Use of vancomycin or first-generation cephalosporins for the treatment of hemodialysis-dependent patients with methicillin-susceptible Staphylococcus aureus bacteremia. Clin Infect Dis 44:190-6
Aksoy, Olcay; Sexton, Daniel J; Wang, Andrew et al. (2007) Early surgery in patients with infective endocarditis: a propensity score analysis. Clin Infect Dis 44:364-72
Fowler Jr, Vance G; Nelson, Charlotte L; McIntyre, Lauren M et al. (2007) Potential associations between hematogenous complications and bacterial genotype in Staphylococcus aureus infection. J Infect Dis 196:738-47

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