Negative selection is the major mechanism of central tolerance by which autoreactive T cells are purged from the repertoire. This process depends upon presentation of self-peptides to developing thymocytes by antigen-presenting cells (APCs). Although the question of which APC is responsible for negative selection has been investigated extensively, the role of thymic B cells as APCs in negative selection has been largely neglected. Among bone marrow-derived APCs, which include dendritic cells (DCs), B cells and macrophages, DCs are ascribed to mediate negative selection based on in vitro assays that compare the efficiency of antigen presentation by these different cell populations. Nevertheless, while B cells are poor at presenting antigens via non-specific uptake, they can present antigens that bind to their B cell receptors (BCR) with even higher efficiency than DCs. Our preliminary studies indicate that antigen-specific thymic B cells are excellent APCs for T cell negative selection. Although the presence of B cells in the thymus has been recognized for several decades, their function is not well understood. They are concentrated in the medulla and cortical-medullary junction along with DCs and express high levels of MHC class II. The capacity of thymic B cells to delete thymocytes has been demonstrated in a superantigen model. It remains unclear whether they can mediate negative selection for cognate antigens recognized by BCRs and what their influence on the T cell repertoire is. We have been investigating the role of thymic B cells in negative selection using a system derived from the K/BxN mouse model of inflammatory arthritis, in which both transgene-encoded T cell receptor (TCR) and BCR are specific for the same glucose-6-phosphate isomerase (GPI) self-antigen. Based on our preliminary results, we hypothesize that thymic B cells are efficient APCs in mediating negative selection of T cells for antigens that are recognized by their BCRs and as such contribute to shaping the T cell repertoire. Given the high prevalence of auto- and polyreactive B cell specificities in the immature B cell repertoire, it is likely that B cells within the thymus are capable of presenting a broad range of self-antigens to developing thymocytes. Thus B cell-mediated antigen presentation could represent a novel pathway for negative selection and maintaining T cell tolerance. The insights obtained on the mechanisms of this process may help develop effective antigen- specific therapy for autoimmune diseases. We will pursue the following specific aims: 1) Determine the capacity of antigen-specific thymic B cells in T cell negative selection;2) Determine the role of thymic B cells in shaping the T cell repertoire;and 3) Determine the contribution of antigen-specific B cells in inducing central versus peripheral T cell tolerance.
Negative selection purges autoreactive T cells from the repertoire. We propose to study the role of antigen specific thymic B cells on T cell negative selection. Better understanding of the mechanisms of this process may help develop effective antigen-specific therapy for autoimmune diseases.
|Bradley, C Pierce; Teng, Fei; Felix, Krysta M et al. (2017) Segmented Filamentous Bacteria Provoke Lung Autoimmunity by Inducing Gut-Lung Axis Th17 Cells Expressing Dual TCRs. Cell Host Microbe 22:697-704.e4|
|Hou, Lifei; Huang, Haochu (2016) Immune suppressive properties of artemisinin family drugs. Pharmacol Ther 166:123-7|
|Block, Katharine E; Zheng, Zhong; Dent, Alexander L et al. (2016) Gut Microbiota Regulates K/BxN Autoimmune Arthritis through Follicular Helper T but Not Th17 Cells. J Immunol 196:1550-7|
|Cai, Liquan; Fisher, Alfred L; Huang, Haochu et al. (2016) CRISPR-mediated genome editing and human diseases. Genes Dis 3:244-251|
|Perera, Jason; Zheng, Zhong; Li, Shuyin et al. (2016) Self-Antigen-Driven Thymic B Cell Class Switching Promotes T Cell Central Tolerance. Cell Rep 17:387-398|
|Perera, Jason; Huang, Haochu (2015) The development and function of thymic B cells. Cell Mol Life Sci 72:2657-63|
|Hou, Lifei; Block, Katharine E; Huang, Haochu (2014) Artesunate abolishes germinal center B cells and inhibits autoimmune arthritis. PLoS One 9:e104762|
|Shilling, Rebecca A; Williams, Jesse W; Perera, Jason et al. (2013) Autoreactive T and B cells induce the development of bronchus-associated lymphoid tissue in the lung. Am J Respir Cell Mol Biol 48:406-14|
|Haggart, Ryan; Perera, Jason; Huang, Haochu (2013) Cloning of a hamster anti-mouse CD79B antibody sequences and identification of a new hamster immunoglobulin lambda constant IGLC gene region. Immunogenetics 65:473-8|
|Perera, Jason; Meng, Liping; Meng, Fanyong et al. (2013) Autoreactive thymic B cells are efficient antigen-presenting cells of cognate self-antigens for T cell negative selection. Proc Natl Acad Sci U S A 110:17011-6|
Showing the most recent 10 out of 12 publications