TITLE: Probing the mechanistic basis for T cell fate decisions. CD4+ T cells use highly sensitive and specific modular biosensors to survey the body for microbial pathogens or tumors. The TCR is the chief mediator of this behavior. It surveys the contents of MHC on antigen presenting cells for peptides (pMHC) derived from microbial pathogens or tumors, and relays pMHC-specific information across the cell membrane to the ten immunoreceptor tyrosine-based activation motifs (ITAMs) of the associated CD3?? , CD3??, and CD3?? signaling modules. CD4 recruits Lck to the TCR-CD3 complex upon concurrent binding of MHC. The quantity and quality of ITAM phosphorylation by Lck provides the base set of instructions that inform CD4+ T cell fate decision. Yet, how the individual subunits of the TCR-CD3-pMHC-CD4 macro-complex fit and work together to drive CD4+ T cell fate decisions remains to be fully defined. Our working hypothesis is that these molecules operate on a similar mechanistic principle to less complex receptor systems, such as cytokine receptors, whereby receptor-associated intracellular signaling enzymes and their substrates are held in a spatial relationship that represents ?off?; pMHC-engagement and reciprocal extracellular interactions between the TCR-CD3 complex and CD4 then positions the intracellular signaling domains in the appropriate spatial relationship for a sufficient duration to initiate and potentiate signaling. Our overarching goal is understand the inner workings of this complex molecular machinery so that we can modify or imitate its form and function to design novel modular biosensors with unique therapeutic functions. During the previous funding period we built multiple experimental platforms to study the spatial relationship between the juxtamembrane (JM) regions of the TCR-CD3 subunits. These allowed us to report the identification of a mechanical switch that relays pMHC-specific information from the TCR-pMHC interface across the T cell membrane to the cytosolic juxtamembrane regions of the CD3?? signaling module. In addition, we performed the first experimental analysis of the architecture of the TCR-CD3-pMHC-CD4 macrocomplex and found that the CD4 JM region is proximal to the CD3 heterodimers, while CD3?? resides on the opposite side of the TCR. We also identified highly conserved residues in the TMD and extracellular domains of CD4 that are important for CD4's Lck-independent and Lck-dependent functions. Finally, we obtained functional evidence for TCR- intrinsic specificity for MHC that we interpret as evidence for MHC scanning. The goals for this renewal application are to deconstruct the molecular mechanisms by which the TCR-CD3-pMHC-CD4 macrocomplex operates and characterize the consequences of these mechanisms in vivo. Our work will yield fundamental insights into the key determinants of CD4+ T cell fate decisions and provide a blueprint for the development of novel modular biosensors with translational potential.

Public Health Relevance

This proposal is focused on increasing our understanding of: (i) how the TCR-CD3 complex and CD4 come together as subunits of a macrocomplex to decode information embedded in a pMHC surface; (ii) how this information is converted to signals at the ITAMs; and (iii) how these mechanisms impact T cell responses (e.g. development, activation, differentiation, effector functions). This work is intended to advance our basic understanding of T cell biology, identify potential targets for the development of immunotherapeutic reagents, and provide the information needed to engineer novel modular biosensors for therapeutic applications.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI101053-09
Application #
10088367
Study Section
Cellular and Molecular Immunology - B Study Section (CMIB)
Program Officer
Mallia, Conrad M
Project Start
2012-08-10
Project End
2022-01-31
Budget Start
2021-02-01
Budget End
2022-01-31
Support Year
9
Fiscal Year
2021
Total Cost
Indirect Cost
Name
University of Arizona
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
806345617
City
Tucson
State
AZ
Country
United States
Zip Code
85721
Glassman, Caleb R; Parrish, Heather L; Lee, Mark S et al. (2018) Reciprocal TCR-CD3 and CD4 Engagement of a Nucleating pMHCII Stabilizes a Functional Receptor Macrocomplex. Cell Rep 22:1263-1275
Lichauco, Katrina; Lee, Mark S; Kuhns, Michael S (2018) Bonds Voyage! A Dissociative Model of TCR-CD3 Triggering Is Proposed. Immunity 49:786-788
Deshpande, Neha R; Uhrlaub, Jennifer L; Way, Sing Sing et al. (2018) A disconnect between precursor frequency, expansion potential, and site-specific CD4+ T cell responses in aged mice. PLoS One 13:e0198354
Parrish, Heather L; Deshpande, Neha R; Vasic, Jelena et al. (2016) Functional evidence for TCR-intrinsic specificity for MHCII. Proc Natl Acad Sci U S A 113:3000-5
Glassman, Caleb R; Parrish, Heather L; Deshpande, Neha R et al. (2016) The CD4 and CD3?? Cytosolic Juxtamembrane Regions Are Proximal within a Compact TCR-CD3-pMHC-CD4 Macrocomplex. J Immunol 196:4713-22
Bethune, Michael T; Gee, Marvin H; Bunse, Mario et al. (2016) Domain-swapped T cell receptors improve the safety of TCR gene therapy. Elife 5:
Parrish, Heather L; Glassman, Caleb R; Keenen, Madeline M et al. (2015) A Transmembrane Domain GGxxG Motif in CD4 Contributes to Its Lck-Independent Function but Does Not Mediate CD4 Dimerization. PLoS One 10:e0132333
Lee, Mark S; Glassman, Caleb R; Deshpande, Neha R et al. (2015) A Mechanical Switch Couples T Cell Receptor Triggering to the Cytoplasmic Juxtamembrane Regions of CD3??. Immunity 43:227-39