An urgent need exists to prevent the sexual transmission of HIV-1 to women. Worldwide, approximately 70% of all new cases are spread by sexual intercourse, with women more likely to be infected than men. The overall objective of this proposal is to define the role of the female reproductive tract (FRT) in preventing viral transmission and to understand how sex hormones modulate FRT HIV target cells to make women more susceptible to infection during the menstrual cycle. This proposal presents an innovative approach to test the hypothesis that during the window of vulnerability, sex hormones (estradiol, progesterone) and chemical contraceptives (oral preparations, subcutaneous implants, and vaginal rings) enhance FRT HIV-target cell receptor expression and infection/ transmission while decreasing interferon-stimulated gene (ISG) expression. Defined as the period during the menstrual cycle when women are most susceptible to HIV infection due to hormonal changes, the window of vulnerability is the time hypothesized by us when women are most likely to be infected by HIV. This is a novel approach for understanding the nature and mechanisms of HIV infection in the FRT, by testing the hypothesis that during the window, FRT HIV-target cell (CD4+T cells, macrophages, dendritic cells) receptor expression and infection/ transmission increases while interferon-stimulated gene (ISG) expression decreases, thus increasing the risk of HIV infection. This approach has 3 original Aims that will: 1) Define the changes in HIV receptor expression, activation and susceptibility to HIV infection of target cells (CD4+ T cells, macrophages and dendritic cells) in the vagina, cervix and uterus during the menstrual cycle; 2) Examine the direct and indirect effects of sex hormones/contraceptives on HIV receptor expression and HIV-susceptibility of target cells in the FRT; and 3) Determine to what extent sex hormones/contraceptives suppress intracellular ISG anti-HIV activity and increase HIV infection of target cells in the FRT. This study is unique in tht it integrates our understanding of the endocrine system and the immune system throughout the human FRT as it relates directly to the very cells most likely to be infected by HIV. Understanding how sex hormones/contraceptives specifically enhance HIV infection by increasing HIV receptors and decreasing intracellular viral protection will provide a basis of knowledge for developing therapeutic mechanisms to prevent transmission of HIV. It further provides a much needed foundation of information that should accelerate the development of multipurpose prevention technologies designed to provide women with safe, effective means of protecting themselves simultaneously from HIV, other sexually transmitted and reproductive tract infections, and/or unintended pregnancy.
An urgent need exists to prevent the sexual transmission of HIV-1 to women. Worldwide, approximately 70% of all new cases are spread by sexual intercourse, with women more likely to be infected than men. The object of this research is to develop a clear understanding of how sex hormones and chemical contraceptives (oral preparations, subcutaneous implants, and vaginal rings) enhance HIV infection of HIV-target cells (CD4+T cells, macrophages, dendritic cells) in the FRT during the 'window of vulnerability' portion of the menstrual cycle when immune protection is depressed and when HIV infection most likely to occur.
|Patel, Mickey V; Shen, Zheng; Rossoll, Richard M et al. (2018) Estradiol-regulated innate antiviral responses of human endometrial stromal fibroblasts. Am J Reprod Immunol 80:e13042|
|Patel, Mickey V; Shen, Zheng; Wira, Charles R (2018) Poly (I:C) and LPS induce distinct immune responses by ovarian stromal fibroblasts. J Reprod Immunol 127:36-42|
|Barr, Fiona D; Ochsenbauer, Christina; Wira, Charles R et al. (2018) Neutrophil extracellular traps prevent HIV infection in the female genital tract. Mucosal Immunol 11:1420-1428|
|Rodriguez-Garcia, Marta; Fortier, Jared M; Barr, Fiona D et al. (2018) Aging impacts CD103+ CD8+ T cell presence and induction by dendritic cells in the genital tract. Aging Cell 17:e12733|
|Rodriguez-Garcia, Marta; Patel, Mickey V; Shen, Zheng et al. (2017) Tenofovir Inhibits Wound Healing of Epithelial Cells and Fibroblasts from the Upper and Lower Human Female Reproductive Tract. Sci Rep 8:45725|
|Shen, Zheng; Rodriguez-Garcia, Marta; Ochsenbauer, Christina et al. (2017) Characterization of immune cells and infection by HIV in human ovarian tissues. Am J Reprod Immunol 78:|
|Shen, Zheng; Rodriguez-Garcia, Marta; Patel, Mickey V et al. (2017) Hormonal Contraceptives Differentially Suppress TFV and TAF Inhibition of HIV Infection and TFV-DP in Blood and Genital Tract CD4+?T cells. Sci Rep 7:17697|
|Rodriguez-Garcia, M; Shen, Z; Barr, F D et al. (2017) Dendritic cells from the human female reproductive tract rapidly capture and respond to HIV. Mucosal Immunol 10:531-544|
|Shen, Zheng; Rodriguez-Garcia, Marta; Patel, Mickey V et al. (2016) Menopausal status influences the expression of programmed death (PD)-1 and its ligand PD-L1 on immune cells from the human female reproductive tract. Am J Reprod Immunol 76:118-25|
|Wira, Charles R; Rodriguez-Garcia, Marta; Patel, Mickey V (2015) The role of sex hormones in immune protection of the female reproductive tract. Nat Rev Immunol 15:217-30|
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