Chlamydia trachomatis is the cause for the most common bacterial sexually transmitted infection in the United States. Commonly referred to as a silent epidemic, C. trachomatis infections in women are frequently asymptomatic, and often go unnoticed and untreated. The infection can persist for months or years and ultimately cause pelvic inflammatory disease, ectopic pregnancies, and infertility. In order to establish persistent infections, C. trachomatis must subvert both the innate and the adaptive branches of the immune system. A central node that connects adaptive and innate immunity to C. trachomatis is the cytokine gamma-interferon (IFN?). This cytokine is produced by lymphocytes such as T cells of the adaptive immune system and induces cell-intrinsic, innate host defenses against Chlamydia in epithelial cells. In order to overcome IFN?-mediated immunity and replicate inside human epithelial cells, C. trachomatis evolved counterdefenses specifically adapted to its human host. These C. trachomatis counterdefenses and the corresponding repertoire of IFN?-inducible anti-Chlamydia defense programs are poorly characterized. Our goal is to decipher this interplay between IFN?-mediated host defense and chlamydial counterdefense, as such knowledge holds the potential to instruct the design of improved treatment options. To achieve this goal under Aim1, we designed unbiased functional genomics screens to identify human IFN?-inducible genes (ISGs) that exert potent anti-Chlamydia defenses. In parallel we employ defined C. trachomatis mutant libraries in functional bacterial genetic screens to identify C. trachomatis genes that subvert ISG- driven defense pathways. As proof-of-principle we already identified i) human ISGs with not-previously- described anti-Chlamydia activities and ii) C. trachomatis mutants that are hyper-susceptible to IFN?- activated host defenses in human cells. These novel human defense pathways and the corresponding C. trachomatis evasion mechanisms will be characterized through multidisciplinary approaches in Aim1. As an interrelated second aim, we will dissect the role of IFN? in C. trachomatis-induced inflammation, which underlies Chlamydia-associated diseases. Although best known as a potent inducer of cell-intrinsic immunity, IFN? also functions as a critical regulator of inflammation and associated diseases. We discovered that specific members of the superfamily of IFN?-inducible dynamin-like GTPases control Chlamydia-induced activation of inflammasomes, a class of cytosolic immune sensors that drive inflammation. Using organoid and other mouse and human cell culture systems as well as novel in vivo mouse models, we will in Aim2 define the function of IFN?-inducible GTPases in Chlamydia-triggered inflammation and in vivo pathogenesis. These studies will reveal critical insights into the role of IFN? in the immunopathology of Chlamydia infections with the potential to guide the development of new therapeutic or prophylactic treatments or vaccine designs.
Chlamydia is amongst the most common causes of sexually transmitted infections and can lead to pelvic inflammatory disease, life-threatening ectopic pregnancies and infertility in women. Disease is linked to the ability of Chlamydia to establish persistent infections in spite of an inflammatory host response. This research will define how Chlamydia induces inflammation but avoids clearance by human immune programs, and it will thereby pave the way towards the development of novel treatment options and improved vaccine strategies.
|Coers, Jörn; Brown, Hailey M; Hwang, Seungmin et al. (2018) Partners in anti-crime: how interferon-inducible GTPases and autophagy proteins team up in cell-intrinsic host defense. Curr Opin Immunol 54:93-101|
|Finethy, Ryan; Luoma, Sarah; Orench-Rivera, Nichole et al. (2017) Inflammasome Activation by Bacterial Outer Membrane Vesicles Requires Guanylate Binding Proteins. MBio 8:|
|Biering, Scott B; Choi, Jayoung; Halstrom, Rachel A et al. (2017) Viral Replication Complexes Are Targeted by LC3-Guided Interferon-Inducible GTPases. Cell Host Microbe 22:74-85.e7|
|Coers, Jörn (2017) Sweet host revenge: Galectins and GBPs join forces at broken membranes. Cell Microbiol 19:|
|Feeley, Eric M; Pilla-Moffett, Danielle M; Zwack, Erin E et al. (2017) Galectin-3 directs antimicrobial guanylate binding proteins to vacuoles furnished with bacterial secretion systems. Proc Natl Acad Sci U S A 114:E1698-E1706|
|Piro, Anthony S; Hernandez, Dulcemaria; Luoma, Sarah et al. (2017) Detection of Cytosolic Shigella flexneri via a C-Terminal Triple-Arginine Motif of GBP1 Inhibits Actin-Based Motility. MBio 8:|
|Zwack, Erin E; Feeley, Eric M; Burton, Amanda R et al. (2017) Guanylate Binding Proteins Regulate Inflammasome Activation in Response to Hyperinjected Yersinia Translocon Components. Infect Immun 85:|
|Schmidt, Elyse A; Fee, Brian E; Henry, Stanley C et al. (2017) Metabolic Alterations Contribute to Enhanced Inflammatory Cytokine Production in Irgm1-deficient Macrophages. J Biol Chem 292:4651-4662|
|Sixt, Barbara S; Bastidas, Robert J; Finethy, Ryan et al. (2017) The Chlamydia trachomatis Inclusion Membrane Protein CpoS Counteracts STING-Mediated Cellular Surveillance and Suicide Programs. Cell Host Microbe 21:113-121|
|Pilla-Moffett, Danielle; Barber, Matthew F; Taylor, Gregory A et al. (2016) Interferon-Inducible GTPases in Host Resistance, Inflammation and Disease. J Mol Biol 428:3495-513|
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