Understanding the molecular basis for why viruses cause disease is an essential requirement for future development of antiviral therapeutics. While we know a great deal about how viruses are recognized and the various ways in which they are combated, there are still vast areas of cellular antiviral immunity that remain unknown. This research proposal focuses on one such area. The central question being addressed in this proposal is whether small RNAs, or components of the mammalian small RNA machinery, contribute to the cellular response to infection. The importance of this question and the need to address it experimentally can be justified through extensive preliminary data that suggest a novel means to combat virus infection is present in mammalian cells but awaits characterization. In an effort to understand the interplay between the small RNA pathway of mammals and the response to virus infection we have discovered the potency by which the RNA induced silencing complex (RISC) can inhibit virus replication, isolated the first mammalian virus protein which inhibits RISC function, and found that Drosha, a key player in small RNA biogenesis, actively translocates to the cytoplasm during RNA virus infection. Taken together, we believe this research provides compelling evidence for an unappreciated small RNA-mediated antiviral response in mammals. Here we propose to use the virus antagonist to RISC (called VP55) to define the small RNA response to infection in vivo as well as characterize how Drosha participates in this antiviral activity. The overall objective of this proposal is to delineate howthe small RNA pathway contributes to our response to virus infection.

Public Health Relevance

Greater understanding of the factors that determine the pathogenicity of viruses is of fundamental importance for the development of future therapeutics. This proposal focuses on characterizing the use of small RNAs and the small RNA machinery in mammals as it relates to our capacity to inhibit virus replication.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI110575-04
Application #
9390016
Study Section
Virology - B Study Section (VIRB)
Program Officer
Park, Eun-Chung
Project Start
2014-12-01
Project End
2019-11-30
Budget Start
2017-12-01
Budget End
2018-11-30
Support Year
4
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Icahn School of Medicine at Mount Sinai
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
078861598
City
New York
State
NY
Country
United States
Zip Code
10029
Møller, Rasmus; Schwarz, Toni M; Noriega, Vanessa M et al. (2018) miRNA-mediated targeting of human cytomegalovirus reveals biological host and viral targets of IE2. Proc Natl Acad Sci U S A 115:1069-1074
Aguado, Lauren C; Jordan, Tristan X; Hsieh, Emily et al. (2018) Homologous recombination is an intrinsic defense against antiviral RNA interference. Proc Natl Acad Sci U S A 115:E9211-E9219
Aguado, Lauren C; Schmid, Sonja; May, Jared et al. (2017) RNase III nucleases from diverse kingdoms serve as antiviral effectors. Nature 547:114-117
Benitez, Asiel A; Panis, Maryline; Xue, Jia et al. (2015) In Vivo RNAi Screening Identifies MDA5 as a Significant Contributor to the Cellular Defense against Influenza A Virus. Cell Rep 11:1714-26
Aguado, Lauren C; Schmid, Sonja; Sachs, David et al. (2015) microRNA Function Is Limited to Cytokine Control in the Acute Response to Virus Infection. Cell Host Microbe 18:714-22
Benitez, Asiel Arturo; Spanko, Laura Adrienne; Bouhaddou, Mehdi et al. (2015) Engineered Mammalian RNAi Can Elicit Antiviral Protection that Negates the Requirement for the Interferon Response. Cell Rep 13:1456-1466
tenOever, Benjamin R (2014) Response to Voinnet et al. Cell Rep 9:798-9