Prevalence of allergic disease continues to rise worldwide. An estimated 40% of the population is sensitized, i.e. have IgE antibodies to foreign proteins in the environment. More worrisome, 40%-50% of school-going children worldwide are getting sensitized to one or more allergens. For example, about 6 million children in the United States suffer from food allergies against peanut, milk, egg, shellfish etc. Other forms of sensitizing allergens can include a multitude of foreign proteins derived from pollens, insects, dust mites, and vegetation, which can manifest as different diseases including allergic rhinitis (hay fever), sinusitis, asthma, and skin reactions. One might even consider allergy as the emerging epidemic of the industrialized world. 'Allergen- specific immunotherapy (ASI)' is the only disease-modifying treatment available, which can desensitize humans to their allergen. Subcutaneous immunotherapy (SCIT) has been the standard for performing ASI, and it involves repeated subcutaneous injection of the allergen to reorient the abnormal cellular and humoral immune responses. SCIT has several drawbacks, including the need for repeated allergen injections over multiple years, the need to be in a clinic to receive the treatment, and a risk of life-threatening systemic anaphylactic reaction. The goal of this proposed research is to develop a novel minimally-invasive cutaneous immunotherapy (CIT) using allergen-coated microneedles (MNs). MNs comprise of microscopic projections on a patch that can be self-applied on the skin, and could take the fear, pain, and inconvenience out of allergy immunotherapy. Our preliminary data presented in this proposal shows that MN-CIT is at least as effective as SCIT.
Our specific aims are: (i) Develop monoallergen-coated MNs and evaluate their short and long term therapeutic effectiveness relative to SCIT, (ii) Develop microneedles for multiallergen immunotherapy and establish their efficacy relative to SCIT, and (iii) Characterize mechanism of MN-CIT and establish that MN-CIT is allergen-specific. If successful these studies will provide data necessary to support further development of microneedles for painless allergy immunotherapy.

Public Health Relevance

This project focuses on development of a novel allergy immunotherapy approach based on microneedles. Microneedles are painless, and convenient to use. Successful completion of the project may provide a patient friendly and simple approach to treat allergies, replacing the current rather prolonged and painful procedure of giving allergy shots with injections.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI121322-02
Application #
9185940
Study Section
Gene and Drug Delivery Systems Study Section (GDD)
Program Officer
Dong, Gang
Project Start
2015-12-01
Project End
2020-11-30
Budget Start
2016-12-01
Budget End
2017-11-30
Support Year
2
Fiscal Year
2017
Total Cost
Indirect Cost
Name
Texas Tech University
Department
Engineering (All Types)
Type
Biomed Engr/Col Engr/Engr Sta
DUNS #
041367053
City
Lubbock
State
TX
Country
United States
Zip Code
79409
Shakya, Akhilesh Kumar; Lee, Chang Hyun; Gill, Harvinder Singh (2018) Coated microneedle-based cutaneous immunotherapy prevents Der p 1-induced airway allergy in mice. J Allergy Clin Immunol 142:2007-2011.e3
Shakya, Akhilesh Kumar; Lee, Chang Hyun; Gill, Harvinder Singh (2017) Cutaneous vaccination with coated microneedles prevents development of airway allergy. J Control Release 265:75-82