Ticks are obligate blood-feeding arthropods that transmit numerous pathogens to both humans and livestock. Lyme disease, which is caused by transmission of the spirochete Borrelia burgdorferi by the deer tick Ixodes scapularis, is responsible for over 95% of vector-borne disease cases in the United States. Despite the importance of ticks as disease vectors, elucidating the molecular basis for tick?pathogen relationships has lagged due to our incomplete understanding of tick innate immunity. We recently identified an antibacterial enzyme in I. scapularis that was co-opted from bacteria through horizontal gene transfer. This domesticated amidase effector (dae2) gene encodes an active, bacteriolytic cell wall hydrolase that is enriched in the gut and salivary glands of ticks. We found that Dae2 affords previously uncharacterized antibacterial activity to the innate immune system of ticks and propose to investigate its role in shaping tick?microbe interactions and vector competence for the Lyme disease pathogen B. burgdorferi. This work will advance our fundamental understanding of how ticks have evolved to selectively control bacteria they transmit and potentially offer new avenues for blocking the tick-borne cycle of infection for an important human pathogen.

Public Health Relevance

Ticks are blood-feeding arthropods that transmit most zoonotic diseases in the United States. Although interactions between ticks and bacterial pathogens can be highly specific, very little is known about vector host factors that modulate tick?microbe interactions. This proposal aims to define the mechanism of a novel antibacterial effector of the innate immune system of the deer tick Ixodes scapularis, which transmits the causative bacterial agent of Lyme borreliosis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI132851-02
Application #
9849725
Study Section
Host Interactions with Bacterial Pathogens Study Section (HIBP)
Program Officer
Ilias, Maliha R
Project Start
2019-01-14
Project End
2023-12-31
Budget Start
2020-01-01
Budget End
2020-12-31
Support Year
2
Fiscal Year
2020
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Biochemistry
Type
Schools of Medicine
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94118