Abstract

We are attempting to extend our analysis of the regulation of glnA expression in K. aerogenes. We are isolating strains carrying the lac genes of E. coli fused to the glnA and glnG promoters in the wild type strain and in mutants with altered glnA expression due to mutations in the glnA region. The expression of these genes can then be studied in the presence and absence of their products. We are attempting to determine the manner of regulation of the asparagine synthetases coded by asnA and asnB. We are continuing our attempt to obtain mutants of B subtilis defective in catabolite repression.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
2R01AM013894-16
Application #
3150889
Study Section
Biochemistry Study Section (BIO)
Project Start
1974-11-01
Project End
1990-03-31
Budget Start
1985-07-01
Budget End
1986-03-31
Support Year
16
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code

  Publications

Magasanik, B (1989) Regulation of transcription of the glnALG operon of Escherichia coli by protein phosphorylation. Biochimie 71:1005-12
Reitzer, L J; Movsas, B; Magasanik, B (1989) Activation of glnA transcription by nitrogen regulator I (NRI)-phosphate in Escherichia coli: evidence for a long-range physical interaction between NRI-phosphate and RNA polymerase. J Bacteriol 171:5512-22
Magasanik, B (1988) Reversible phosphorylation of an enhancer binding protein regulates the transcription of bacterial nitrogen utilization genes. Trends Biochem Sci 13:475-9
Courchesne, W E; Magasanik, B (1988) Regulation of nitrogen assimilation in Saccharomyces cerevisiae: roles of the URE2 and GLN3 genes. J Bacteriol 170:708-13
Weiss, V; Magasanik, B (1988) Phosphorylation of nitrogen regulator I (NRI) of Escherichia coli. Proc Natl Acad Sci U S A 85:8919-23
Magasanik, B (1988) Research on bacteria in the mainstream of biology. Science 240:1435-9
Ninfa, A J; Reitzer, L J; Magasanik, B (1987) Initiation of transcription at the bacterial glnAp2 promoter by purified E. coli components is facilitated by enhancers. Cell 50:1039-46
Reitzer, L J; Magasanik, B (1986) Transcription of glnA in E. coli is stimulated by activator bound to sites far from the promoter. Cell 45:785-92
Ninfa, A J; Magasanik, B (1986) Covalent modification of the glnG product, NRI, by the glnL product, NRII, regulates the transcription of the glnALG operon in Escherichia coli. Proc Natl Acad Sci U S A 83:5909-13
Ninfa, A J; Ueno-Nishio, S; Hunt, T P et al. (1986) Purification of nitrogen regulator II, the product of the glnL (ntrB) gene of Escherichia coli. J Bacteriol 168:1002-4

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