Abstract

The objective of the project is to study the mechanisms of action of parathyroid hormone (PTH) focusing primarily on the role of cAMP, and the development of a bioassay based on stimulation of adenylate cyclase from osseous tissue. For establishing the PTH bioassay based on stimulation of osseous tissue adenylate cyclase, we propose to characterize the adenylate cyclase of the highly responsive osteosarcoma clone (ROS 17/2) with respect to: optimum assay conditions for maximizing hormone response (MgATP2-, Mg2ion, GTP) and compare effects of PTH from various species as well as synthetic PTH fragments and analogs. We propose to investigate the role of the previously dscovered adenylate cyclase enhancing cytosol factor (Egan et al., BBRC 80:176-182, 1978) on hormone stimulation of the enzyme using: (1) responsive and non responsive clones and (2) the parallel in vivo inducibility of cytosol factor and hormone response, as experimental tools.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM017848-11
Application #
3151096
Study Section
General Medicine B Study Section (GMB)
Project Start
1978-08-01
Project End
1987-06-30
Budget Start
1985-08-01
Budget End
1987-06-30
Support Year
11
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Connecticut
Department
Type
Schools of Dentistry/Oral Hygn
DUNS #
City
Farmington
State
CT
Country
United States
Zip Code