Abstract

This research project is concerned with the study of autoantibodies in the sera of patients with connective tissue diseases. These autoantibodies arise spontaneously in patients with systemic lupus erythematosus, mixed connective tissue disease, Sjogren's syndrome and scleroderma. Many of these antibodies react specifically with non-histone proteins complexed to chromosomes. One of the objectives is to characterize each nuclear antigen-antibody system. Information obtained to date has shown that antibodies to different non-histone proteins are present in different disease conditions. In other words, diseases such as systemic lupus erythematosus or scleroderma have their own characteristic profiles of antibodies to nuclear non-histone proteins. Thus, characterization of the specificities of these antibodies can be used as markers in differential diagnosis. The naturally occurring autoantibodies have also been used as reagents to isolate nuclear non-histone proteins. We have characterized a centromere (kinetochore) antigen with the use of one of these reagent antibodies. Work in progress is currently concerned with the isolation and characterization of other non-histone chromosomal proteins which react with autoimmune antibodies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM032063-04
Application #
3152412
Study Section
Immunological Sciences Study Section (IMS)
Project Start
1983-01-01
Project End
1987-12-31
Budget Start
1985-01-01
Budget End
1985-12-31
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Scripps Research Institute
Department
Type
DUNS #
City
San Diego
State
CA
Country
United States
Zip Code
92037

  Publications

Chan, E K; Sullivan, K F; Tan, E M (1989) Ribonucleoprotein SS-B/La belongs to a protein family with consensus sequences for RNA-binding. Nucleic Acids Res 17:2233-44
Reimer, G; Raska, I; Scheer, U et al. (1988) Immunolocalization of 7-2-ribonucleoprotein in the granular component of the nucleolus. Exp Cell Res 176:117-28
Ben-Chetrit, E; Chan, E K; Sullivan, K F et al. (1988) A 52-kD protein is a novel component of the SS-A/Ro antigenic particle. J Exp Med 167:1560-71
Reimer, G; Steen, V D; Penning, C A et al. (1988) Correlates between autoantibodies to nucleolar antigens and clinical features in patients with systemic sclerosis (scleroderma). Arthritis Rheum 31:525-32
Reimer, G; Pollard, K M; Penning, C A et al. (1987) Monoclonal autoantibody from a (New Zealand black x New Zealand white)F1 mouse and some human scleroderma sera target an Mr 34,000 nucleolar protein of the U3 RNP particle. Arthritis Rheum 30:793-800
Tsay, G J; Chan, E K; Peebles, C L et al. (1987) An immunoassay differentiating sera with antibodies to Sm alone, antibodies to Sm/RNP complex, and antibodies to RNP alone. Arthritis Rheum 30:389-96
Pollard, K M; Chan, E K; Rubin, R L et al. (1987) Monoclonal autoantibodies to nuclear antigens from murine graft-versus-host disease. Clin Immunol Immunopathol 44:31-40
Reimer, G; Rose, K M; Scheer, U et al. (1987) Autoantibody to RNA polymerase I in scleroderma sera. J Clin Invest 79:65-72
Tan, E M; Reimer, G; Sullivan, K (1987) Intracellular autoantigens: diagnostic fingerprints but aetiological dilemmas. Ciba Found Symp 129:25-42
Waga, S; Tan, E M; Rubin, R L (1987) Identification and isolation of soluble histones from bovine milk and serum. Biochem J 244:675-82

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