The specific aims of this application are to continue the investigation of factors influencing the principles of vascularized bone grafts. Currently available vascularized bone grafts used to reconstruct massive segmental bone loss in compromised beds following wide resections of malignant bone tumors, traumatic bony defects, and infected non-unions have significant limitations resulting from structural confirmation of the graft, and donor site morbidity. The central goal of this proposal is to utilize biosynthetic components (hydroxyapatite ceramic mold), osteoinductive principles and osteoprogenitor cells coupled with a vascularized pedicle to produce """"""""a molded vascularized osteogenesis"""""""". The second goal of this proposal is to isolate bone marrow stromal osteoprogenitor cells, determine the appropriate degree of differentiation that maximizes osteogenesis in vivo and expand this to defined populations ex vivo prior to utilization in molded osteogenesis. Studies have been designed to isolate the characterize osteoprogenitor cells from rabbit marrow in vitro and utilize diffusion chambers to determine the appropriate level of contact with matrix host cells and to determine the effects of demineralized bone matrix and of growth factors on heterotopic bone regeneration. This vascularized molded bone graft will be used as an island graft or a free transfer and the healing and material properties of these grafts will be examined and compared to conventional bone grafts.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
2R01AR025791-12A2
Application #
3155352
Study Section
Orthopedics and Musculoskeletal Study Section (ORTH)
Project Start
1979-07-01
Project End
1993-12-31
Budget Start
1993-01-01
Budget End
1993-12-31
Support Year
12
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Hospital for Special Surgery
Department
Type
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10021
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Bostrom, M; Lane, J M; Tomin, E et al. (1996) Use of bone morphogenetic protein-2 in the rabbit ulnar nonunion model. Clin Orthop Relat Res :272-82
Mizumoto, S; Inada, Y; Weiland, A J (1992) Pre-formed vascularized bone grafts using polyethelyne chambers. J Reconstr Microsurg 8:325-33
Yasko, A W; Lane, J M; Fellinger, E J et al. (1992) The healing of segmental bone defects, induced by recombinant human bone morphogenetic protein (rhBMP-2). A radiographic, histological, and biomechanical study in rats. J Bone Joint Surg Am 74:659-70