Abstract

Through the analysis of selected idiotypes, we hope to continue our characterization of anti-DNA antibodies in the human disease, systemic lupus erythematosus, and in two murine models. New anti-idiotypes will be generated to identify the structural basis for DNA binding and to characterize subsets of anti-DNA antibodies that differ in pathogenicity. We will generate idiotype positive anti-DNA antibody producing cell lines from SLE patients and from autoimmune mice. Using these lines, we will determine whether there are germ line immunoglobulin variable region genes that encode anti-DNA antibodies or whether anti-DNA antibodies are the consequence of somatic mutation in germ line immunoglobulin genes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR032371-08
Application #
3156270
Study Section
Immunological Sciences Study Section (IMS)
Project Start
1983-04-01
Project End
1991-03-31
Budget Start
1990-04-01
Budget End
1991-03-31
Support Year
8
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Type
Schools of Medicine
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Chu, Yih-Pai; Taylor, Devon; Yan, Han-Guang et al. (2002) Persistence of partially functional double-stranded (ds) DNA binding B cells in mice transgenic for the IgM heavy chain of an anti-dsDNA antibody. Int Immunol 14:45-54
Lee, H B; Diamond, B A; Blaufox, M D (2001) In vivo detection of deposition of radiolabeled lupus antikidney antibody and its inhibition by soluble antigen. J Nucl Med 42:138-40
Davidson, A; Diamond, B (2001) Autoimmune diseases. N Engl J Med 345:340-50
Kowal, C; Weinstein, A; Diamond, B (1999) Molecular mimicry between bacterial and self antigen in a patient with systemic lupus erythematosus. Eur J Immunol 29:1901-11
Manheimer-Lory, A J; Zandman-Goddard, G; Davidson, A et al. (1997) Lupus-specific antibodies reveal an altered pattern of somatic mutation. J Clin Invest 100:2538-46
Gaynor, B; Putterman, C; Valadon, P et al. (1997) Peptide inhibition of glomerular deposition of an anti-DNA antibody. Proc Natl Acad Sci U S A 94:1955-60
Irigoyen, M; Kowal, C; Young, A C et al. (1996) Molecular mapping of the 8.12 SLE-associated idiotype specificity at the single amino acid level. Mol Immunol 33:1255-65
Irigoyen, M; Manheimer-Lory, A; Gaynor, B et al. (1994) Molecular analysis of the human immunoglobulin V lambda II gene family. J Clin Invest 94:532-8
Katz, J B; Limpanasithikul, W; Diamond, B (1994) Mutational analysis of an autoantibody: differential binding and pathogenicity. J Exp Med 180:925-32
Iliev, A; Spatz, L; Ray, S et al. (1994) Lack of allelic exclusion permits autoreactive B cells to escape deletion. J Immunol 153:3551-6

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