Etiology of Rheumatoid Arthritis (RA) and Sle

Steinman, Charles R.

Abstract

The proposed research project focuses on studies designed to elucidate basic causal mechanisms in systemic lupus erythematosus and related disorders. By the application of recently devised techniques based on advances in nucleic acid chemistry and molecular biology, a detailed analysis of circulating DNA in both normal individuals and SLE patients is proposed. This analysis is designed to elucidate basic causal mechanisms including possible causal involvement of microorganisms in these diseases. The use of recombinant DNA methodology will allow this work to be greatly faciliated over that previously carried out in this laboratory. Other studies relevant to the role of extranuclear DNA and antibodies to it will also be pursued as will studies designed to identify putative causative microorganisms in these diseases.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
• Type: Research Project (R01)
• Project #: 5R01AR033278-06
• Application #: 3156552
• Study Section: General Medicine A Subcommittee 2 (GMA)
• Project Start: 1983-02-01
• Project End: 1989-08-31
• Budget Start: 1987-09-01
• Budget End: 1989-08-31
• Support Year: 6
• Fiscal Year: 1987

Institution

• Name: Suny Downstate Medical Center
• Type: Schools of Medicine
• DUNS #: 068552207
• City: Brooklyn
• State: NY
• Country: United States
• Zip Code: 11203

Publications

Yu, D; Mukai, M; Liu, Q et al. (1997) Specific inhibition of PCR by non-extendable oligonucleotides using a 5' to 3' exonuclease-deficient DNA polymerase. Biotechniques 23:714-6, 718-20

Steinman, C R; Muralidhar, B; Nuovo, G J et al. (1997) Domain-directed polymerase chain reaction capable of distinguishing bacterial from host DNA at the single-cell level: characterization of a systematic method to investigate putative bacterial infection in idiopathic disease. Anal Biochem 244:328-39

Muralidhar, B; Steinman, C R (1994) Design and characterization of PCR primers for detection of pathogenic Neisseriae. Mol Cell Probes 8:55-61

Muralidhar, B; Rumore, P M; Steinman, C R (1994) Use of the polymerase chain reaction to study arthritis due to Neisseria gonorrhoeae. Arthritis Rheum 37:710-7

Muralidhar, B; Steinman, C R (1992) Geometric differences allow differential enzymatic inactivation of PCR product and genomic targets. Gene 117:107-12

Rumore, P; Muralidhar, B; Lin, M et al. (1992) Haemodialysis as a model for studying endogenous plasma DNA: oligonucleosome-like structure and clearance. Clin Exp Immunol 90:56-62

Chen, K; Neimark, H; Rumore, P et al. (1989) Broad range DNA probes for detecting and amplifying eubacterial nucleic acids. FEMS Microbiol Lett 48:19-24

Li, J Z; Steinman, C R (1989) Plasma DNA in systemic lupus erythematosus. Characterization of cloned base sequences. Arthritis Rheum 32:726-33