Abstract

The major consideration in utilizing island flaps and free flaps of skin and subcutaneous tissue is tissue viability. Superoxide free radicals have been implicated in the etiology of cellular injury in various pathophysiological processes. The present proposal seeks to elucidate the role of superoxide free radicals in the tissue damage which occurs during ischemia and reperfusion. Allopurinol blockade of superoxide generation and scavengers of oxygen free radicals (superoxide dismutase, dimethyl sulfoxide, Alpha-tocopherol) will be investigated in terms of enhanced flap survival. The effect of hyperbaric oxygen on flap survival will be investigated to determine possible induction of antitoxidant enzymes. Additionally, the glutathione peroxidase system and reducing equivalents (NADH, NADPH, GSH) will be investigated. Fundamental knowledge concerning detoxification of free radicals will suggest therapeutic means for minimizing the effects of ischemia. Insights gained with respect to free flaps will prove valuable in the field of allotransplantation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR033638-02
Application #
3156604
Study Section
Surgery, Anesthesiology and Trauma Study Section (SAT)
Project Start
1985-09-20
Project End
1988-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Manson, P N; Jesudass, R; Marzella, L et al. (1991) Evidence for an early free radical-mediated reperfusion injury in frostbite. Free Radic Biol Med 10:7-11
Kaelin, C M; Im, M J; Myers, R A et al. (1990) The effects of hyperbaric oxygen on free flaps in rats. Arch Surg 125:607-9
Im, M J; Lee, W P; Hoopes, J E (1990) Effect of electrical stimulation on survival of skin flaps in pigs. Phys Ther 70:37-40
Kim, Y S; Im, M J; Hoopes, J E (1990) The effect of a free-radical scavenger, N-2-mercaptopropionylglycine, on the survival of skin flaps. Ann Plast Surg 25:18-20
Im, M J; Hoopes, J E; Yoshimura, Y et al. (1989) Xanthine:acceptor oxidoreductase activities in ischemic rat skin flaps. J Surg Res 46:230-4
Yoon, J O; Im, M J; Manson, P N et al. (1989) The role of metal ions in ischemia/reperfusion injury in skin flaps. J Surg Res 46:163-5
Im, M J; Hoopes, J E (1989) Improved skin flap survival with nicotinic acid and nicotinamide in rats. J Surg Res 47:453-5