Abstract

It is proposed to study in depth, the muscle satellite cells, with emphasis on the following objectives: 1. The morphology and distribution of satellite cells in neonatal muscle. 2. Determination of the effect of pathologic processes and trauma (dystrophy and denervation) on the quantity and ultrastructure of muscle satellite cells. 3. Comparison of satellite cell dynamics in myogenesis and growth of dystrophic and normal muscle. 4. Investigation of the origin and fate of satellite cells in degenerating and regenerating muscle. 5. Analysis of the significance of the satellite cell in muscular dystrophy. In vivo studies of two genetic mutant mouse strains (C57BL/6J-dy2J and 129/Re-dy) will be carried out using light and electron microscopy and light microscopic radioautography.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR036294-11
Application #
3157537
Study Section
Neurology B Subcommittee 1 (NEUB)
Project Start
1986-01-01
Project End
1988-12-31
Budget Start
1987-01-01
Budget End
1987-12-31
Support Year
11
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Pittsburgh
Department
Type
Schools of Medicine
DUNS #
053785812
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

DeRosimo, J F; Washabaugh, C H; Ontell, M P et al. (2000) Enhancement of adult muscle regeneration by primary myoblast transplantation. Cell Transplant 9:369-77
Chen, H H; Mack, L M; Choi, S Y et al. (1999) DNA from both high-capacity and first-generation adenoviral vectors remains intact in skeletal muscle. Hum Gene Ther 10:365-73
Floyd Jr, S S; Clemens, P R; Ontell, M R et al. (1998) Ex vivo gene transfer using adenovirus-mediated full-length dystrophin delivery to dystrophic muscles. Gene Ther 5:19-30
Yang, J; Kelly, R; Daood, M et al. (1998) Alteration in myosatellite cell commitment with muscle maturation. Dev Dyn 211:141-52
Chen, H H; Mack, L M; Kelly, R et al. (1997) Persistence in muscle of an adenoviral vector that lacks all viral genes. Proc Natl Acad Sci U S A 94:1645-50
Yang, J; Ontell, M P; Kelly, R et al. (1997) Limitations of nls beta-galactosidase as a marker for studying myogenic lineage or the efficacy of myoblast transfer. Anat Rec 248:40-50
Arcila, M E; Ameredes, B T; DeRosimo, J F et al. (1997) Mass and functional capacity of regenerating muscle is enhanced by myoblast transfer. J Neurobiol 33:185-98
Sopper, M M; Hauschka, S D; Hoffman, E et al. (1994) Gene complementation using myoblast transfer into fetal muscle. Gene Ther 1:108-13
Ontell, M P; Hughes, D; Hauschka, S D et al. (1992) Transient neonatal denervation alters the proliferative capacity of myosatellite cells in dystrophic (129ReJdy/dy) muscle. J Neurobiol 23:407-19
Ontell, M P; Moschella, M C; Schiaffino, S et al. (1992) Modification of the dystrophic phenotype after transient neonatal denervation: role of MHC isoforms. J Neurobiol 23:751-65

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