Abstract

The long-term goal of these studies is to understand the molecular basis of assembly of specific tissue and organ structures by testing the hypothesis that tissue dependent expression of transcript and protein variants determines the specific micro-architecture of the extracellular matrix in different tissues. To reach this goals, we will use a combination of DNA cloning, immunohistochemistry, and transgenic mice technology to analyze the differential expression and interaction properties of the FACIT components IX, XII, and XIV. We will also study the regulated expression and biological role of a novel collagen (type XVII) with multiple triple-helical domains and sequence characteristics typical of proteoglycans. We believe our studies will contribute significantly to the molecular understanding of tissue-dependent diversity of extracellular matrices. This information is essential for understanding normal tissue and organ morphogenesis as well as connective tissue disease processes and genetic abnormalities.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR036820-13
Application #
2442798
Study Section
Pathobiochemistry Study Section (PBC)
Project Start
1985-07-01
Project End
1998-06-30
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
13
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Harvard University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Hu, Kai; Olsen, Bjorn R (2017) Vascular endothelial growth factor control mechanisms in skeletal growth and repair. Dev Dyn 246:227-234
Olsen, Bjorn R; Berendsen, Agnes D; Besschetnova, Tatiana Y et al. (2016) Fell-Muir Lecture: Regulatory mechanisms of skeletal and connective tissue development and homeostasis - lessons from studies of human disorders. Int J Exp Pathol 97:296-302
Agarwal, Shailesh; Loder, Shawn; Brownley, Cameron et al. (2016) Inhibition of Hif1? prevents both trauma-induced and genetic heterotopic ossification. Proc Natl Acad Sci U S A 113:E338-47
Huang, Wei; Li, Qing; Amiry-Moghaddam, Mahmood et al. (2016) Critical Endothelial Regulation by LRP5 during Retinal Vascular Development. PLoS One 11:e0152833
Duan, Xuchen; Bradbury, Seth R; Olsen, Bjorn R et al. (2016) VEGF stimulates intramembranous bone formation during craniofacial skeletal development. Matrix Biol 52-54:127-140
Duan, Xuchen; Murata, Yurie; Liu, Yanqiu et al. (2015) Vegfa regulates perichondrial vascularity and osteoblast differentiation in bone development. Development 142:1984-91
Berendsen, Agnes D; Olsen, Bjorn R (2014) How vascular endothelial growth factor-A (VEGF) regulates differentiation of mesenchymal stem cells. J Histochem Cytochem 62:103-8
Dellinger, Michael T; Garg, Nupur; Olsen, Bjorn R (2014) Viewpoints on vessels and vanishing bones in Gorham-Stout disease. Bone 63:47-52
Duncan, Michael B; Yang, Changqing; Tanjore, Harikrishna et al. (2013) Type XVIII collagen is essential for survival during acute liver injury in mice. Dis Model Mech 6:942-51
Adams, Allysa; McBratney-Owen, Brandeis; Newby, Brittany et al. (2013) Presphenoidal synchondrosis fusion in DBA/2J mice. Mamm Genome 24:54-62

Showing the most recent 10 out of 132 publications