We propose to employ a feline model of a lysosomal storage disease, mucopolysaccharidosis VI [arylsulfatase B (ASB) deficiency], to investigate prospectively the degree to which various clinicopathologic and biochemical manifestations of this glycosaminoglycans (GAG) storage disease are prevented, stabilized or corrected through allogeneic bone marrow transplantation (BMT). We have established a breeding colony of mucopolysaccharidosis VI (MPS VI)-affected (homozygous) and carrier (heterozygous) cats. In our initial studies, we have corrected leukocyte arylsulfatase B deficiency in 3 of 3 cats by BMT. Following bone marrow transplantation, many of the clinical features related to excess GAG storage, e.g. corneal clouding, facial dysmorphia, inability to ambulate, and excess urinary excretion of GAG have been reversed after donor-origin, ASB-competent leukocytes have become engrafted. We propose to extend the above observations by conducting additional allogeneic bone marrow transplants in MPS VI affected cats to determine the mechanism by which BMT may prevent, stabilize, or reverse manifestations of MPS VI and the importance of age of MPS-affected individual at time of BMT in influencing the efficacy of this treatment. We will perform BMT in 3 groups of 5 MPS VI-affected cats each at the ages of 12 weeks, 6 months, and 1 year and monitor the following parameters pre- and post-BMT in cats: Radiography and echocardiography and biomicroscopy to evaluate effects of GAG accumulation in skeletal cardiac, corneal, and other visceral tissues; and light and electron microscopy accumulation to localize the intracellular and interstitial of GAG in tissues and lesions; leukocyte, tissue, and urinary arylsulfatase B activity will be assessed to determine whether engraftment has occurred and the degree to which enzyme-competent cells will reverse the GAC accumulation and lesions in affected target tissues. Five MPS VI-affected not treated by BMT cats will be used as controls. We also propose to determine the effect of MPS VI affected allogeneic bone marrow transplantation into unaffected normal recipients to ascertain the extent to which bone marrow derived cells are responsible for glycosaminoglycans degradation in various tissues and organs. Results from these studies will provide information directly pertinent to therapy of lysosomal storage disorders of humans by bone marrow transplantation.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
1R01AR037095-01
Application #
3157935
Study Section
Metabolic Pathology Study Section (MEP)
Project Start
1986-04-01
Project End
1989-03-31
Budget Start
1986-04-01
Budget End
1987-03-31
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Colorado State University-Fort Collins
Department
Type
Schools of Veterinary Medicine
DUNS #
112617480
City
Fort Collins
State
CO
Country
United States
Zip Code
80523
Walkley, S U; Thrall, M A; Haskins, M E et al. (2005) Abnormal neuronal metabolism and storage in mucopolysaccharidosis type VI (Maroteaux-Lamy) disease. Neuropathol Appl Neurobiol 31:536-44
Dial, S M; Byrne, T; Haskins, M et al. (1997) Urine glycosaminoglycan concentrations in mucopolysaccharidosis VI-affected cats following bone marrow transplantation or leukocyte infusion. Clin Chim Acta 263:1-14
Norrdin, R W; Simske, S J; Gaarde, S et al. (1995) Bone changes in mucopolysaccharidosis VI in cats and the effects of bone marrow transplantation: mechanical testing of long bones. Bone 17:485-9
Turner, A S; Norrdin, R W; Gaarde, S et al. (1995) Bone mineral density in feline mucopolysaccharidosis VI measured using dual-energy X-ray absorptiometry. Calcif Tissue Int 57:191-5
Walkley, S U; Thrall, M A; Dobrenis, K et al. (1994) Bone marrow transplantation corrects the enzyme defect in neurons of the central nervous system in a lysosomal storage disease. Proc Natl Acad Sci U S A 91:2970-4
Norrdin, R W; Moffat, K S; Thrall, M A et al. (1993) Characterization of osteopenia in feline mucopolysaccharidosis VI and evaluation of bone marrow transplantation therapy. Bone 14:361-7
Colgan, S P; Hull-Thrall, M A; Gasper, P W et al. (1991) Restoration of neutrophil and platelet function in feline Chediak-Higashi syndrome by bone marrow transplantation. Bone Marrow Transplant 7:365-74
Lowenthal, A C; Cummings, J F; Wenger, D A et al. (1990) Feline sphingolipidosis resembling Niemann-Pick disease type C. Acta Neuropathol 81:189-97