Extracellular Processing of Cartilage Proteoglycans

Sandy, John D.

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
• Type: Research Project (R01)
• Project #: 7R01AR038580-03
• Application #: 3158645
• Study Section: Pathobiochemistry Study Section (PBC)
• Project Start: 1989-01-01
• Project End: 1992-07-31
• Budget Start: 1989-01-01
• Budget End: 1989-07-31
• Support Year: 3
• Fiscal Year: 1988

Institution

• Name: University of South Florida
• Type: Schools of Medicine
• City: Tampa
• State: FL
• Country: United States
• Zip Code: 33612

Publications

Flannery, C R; Sandy, J D (1994) Effects of proteinase inhibitors on aggrecan catabolism in chondrocyte cultures. Ann N Y Acad Sci 732:380-3

Plaas, A H; Sandy, J D (1993) A cartilage explant system for studies on aggrecan structure, biosynthesis and catabolism in discrete zones of the mammalian growth plate. Matrix 13:135-47

Lohmander, L S; Neame, P J; Sandy, J D (1993) The structure of aggrecan fragments in human synovial fluid. Evidence that aggrecanase mediates cartilage degradation in inflammatory joint disease, joint injury, and osteoarthritis. Arthritis Rheum 36:1214-22

Sandy, J D; Flannery, C R; Neame, P J et al. (1992) The structure of aggrecan fragments in human synovial fluid. Evidence for the involvement in osteoarthritis of a novel proteinase which cleaves the Glu 373-Ala 374 bond of the interglobular domain. J Clin Invest 89:1512-6

Flannery, C; Stanescu, V; Morgelin, M et al. (1992) Variability in the G3 domain content of bovine aggrecan from cartilage extracts and chondrocyte cultures. Arch Biochem Biophys 297:52-60

Flannery, C R; Lark, M W; Sandy, J D (1992) Identification of a stromelysin cleavage site within the interglobular domain of human aggrecan. Evidence for proteolysis at this site in vivo in human articular cartilage. J Biol Chem 267:1008-14

Sandy, J D; Neame, P J; Boynton, R E et al. (1991) Catabolism of aggrecan in cartilage explants. Identification of a major cleavage site within the interglobular domain. J Biol Chem 266:8683-5

Sandy, J D; Boynton, R E; Flannery, C R (1991) Analysis of the catabolism of aggrecan in cartilage explants by quantitation of peptides from the three globular domains. J Biol Chem 266:8198-205

Flannery, C R; Urbanek, P J; Sandy, J D (1990) The effect of maturation and aging on the structure and content of link proteins in rabbit articular cartilage. J Orthop Res 8:78-85

Sandy, J D; Flannery, C R; Boynton, R E et al. (1990) Isolation and characterization of disulfide-bonded peptides from the three globular domains of aggregating cartilage proteoglycan. J Biol Chem 265:21108-13