Abstract

Hybridoma-produced autoantibodies to DNA will be used to analyze genetic and cellular aspects of experimental systemic lupus erythematosus. These monoclonal autoantibodies, together with corresponding anti-idiotypic antibodies will be employed to quantitate cross reactive idiotypes in serum as well as suppressor T cells, helper T cells and B cells with specificity for autoantibodies to DNA. Experiments will be done in MRL/I mice, which develop a severe and rapidly fatal form of systemic lupus erythematosus. The hybridoma autoantibodies will also be used to study recognition of helical nucleic acids by proteins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
7R01AR038820-01
Application #
3158809
Study Section
Immunological Sciences Study Section (IMS)
Project Start
1986-09-01
Project End
1988-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Tufts University
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02111

  Publications

Stollar, B D (1992) Immunochemical analyses of nucleic acids. Prog Nucleic Acid Res Mol Biol 42:39-77
Huang, C; Stollar, B D (1991) Construction of representative immunoglobulin variable region cDNA libraries from human peripheral blood lymphocytes without in vitro stimulation. J Immunol Methods 141:227-36
Stollar, B D; McInerney, T; Gavron, T et al. (1989) Cross-reactions of nucleic acids with monoclonal antibodies to phosphatidylinositol phosphate and cholesterol. Mol Immunol 26:73-9
Zouali, M; Migliorini, P; Mackworth-Young, C G et al. (1988) Nucleic acid-binding specificity and idiotypic expression of canine anti-DNA antibodies. Eur J Immunol 18:923-7
Sabbaga, J; Migliorini, P; Schwartz, R S (1988) Autoantibodies to DNA. In Vivo 2:61-5
Souroujon, M; White-Scharf, M E; Andreschwartz, J et al. (1988) Preferential autoantibody reactivity of the preimmune B cell repertoire in normal mice. J Immunol 140:4173-9
Zouali, M; Migliorini, P; Stollar, D B (1987) Murine lupus anti-DNA antibodies cross-react with the hapten (4-hydroxy-5-iodo-3-nitrophenyl)acetyl, but immunization-induced anti-DNA antibodies do not. Eur J Immunol 17:509-13