Abstract

The investigator has developed a novel approach involving the injection of carbocyanine dyes (diI, di0, diA) into developing chick dermomyotomes to determine the origin and migration of cells giving rise to the myotome. Experiments will be performed to locate potential centers of mitotic activity, precisely locate origin of myogenic precursor cells and their paths of migration, determine origin of precursors of hypaxial myogenic cells, and examine precursor-product relationships.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR044483-03
Application #
2899914
Study Section
Biological Sciences 2 (BIOL)
Program Officer
Lymn, Richard W
Project Start
1997-04-01
Project End
2001-01-31
Budget Start
1999-04-01
Budget End
2001-01-31
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Yang, Yagai; Ordahl, Charles P (2006) The pattern of MyoD and contractile protein localization in primary epaxial myotome reflects the dynamic progression of nascent myoblast differentiation. Dev Dyn 235:382-94
Towler, Mhairi C; Gleeson, Paul A; Hoshino, Sachiko et al. (2004) Clathrin isoform CHC22, a component of neuromuscular and myotendinous junctions, binds sorting nexin 5 and has increased expression during myogenesis and muscle regeneration. Mol Biol Cell 15:3181-95
Kirsten, Eva; Kun, Ernest; Mendeleyev, Jerome et al. (2004) Activity assays for poly-ADP ribose polymerase. Methods Mol Biol 287:137-49
Venters, Sara J; Ordahl, Charles P (2002) Persistent myogenic capacity of the dermomyotome dorsomedial lip and restriction of myogenic competence. Development 129:3873-85
Denetclaw Jr, W F; Berdougo, E; Venters, S J et al. (2001) Morphogenetic cell movements in the middle region of the dermomyotome dorsomedial lip associated with patterning and growth of the primary epaxial myotome. Development 128:1745-55
Ordahl, C P; Berdougo, E; Venters, S J et al. (2001) The dermomyotome dorsomedial lip drives growth and morphogenesis of both the primary myotome and dermomyotome epithelium. Development 128:1731-44
Williams, B A; Ordahl, C P (2000) Fate restriction in limb muscle precursor cells precedes high-level expression of MyoD family member genes. Development 127:2523-36
Dockter, J; Ordahl, C P (2000) Dorsoventral axis determination in the somite: a re-examination. Development 127:2201-6
Denetclaw, W F; Ordahl, C P (2000) The growth of the dermomyotome and formation of early myotome lineages in thoracolumbar somites of chicken embryos. Development 127:893-905
Ordahl, C P; Williams, B A (1998) Knowing chops from chuck: roasting myoD redundancy. Bioessays 20:357-62