Core - Tissue Culture

Abstract

The Tissue Culture Core will support each of the projects by providing cells, technical support and facilities that include: 1. Routine isolation of primary fetal rat calvarial-derived and human derived osteoblasts as needed; 2. Maintenance of the cultures under defined experimental conditions throughout the osteoblast developmental period; 3. Maintenance of osteosarcoma and non-osseous cell lines; 4. Monitoring growth and differentiation parameters that include photography and histochemical detection procedures, cell counts, H- thymidine labelling; 5. Harvesting media and cell layers samples for assays by the Biochemistry and Molecular Biology Cores for specific experiments; 6. Preparation and sterility testing of cell culture media, screening of serum lots and reagents for the culture to ensure uniformity of cultures; 7. Collating parameters of cell growth-differentiation and tissue organization for distribution to project investigations.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
• Type: Research Project (R01)
• Project #: 5R01AR045688-01
• Application #: 2777892
• Budget Start: 1997-10-01
• Budget End: 1998-09-30
• Support Year: 1
• Fiscal Year: 1998

Institution

• Name: University of Massachusetts Medical School Worcester
• DUNS #: 660735098
• City: Worcester
• State: MA
• Country: United States
• Zip Code: 01655

Publications

Ali, Syed A; Zaidi, Sayyed K; Dobson, Jason R et al. (2010) Transcriptional corepressor TLE1 functions with Runx2 in epigenetic repression of ribosomal RNA genes. Proc Natl Acad Sci U S A 107:4165-9

Stein, G S; Stein, J L; Lian, J B et al. (2004) Nuclear microenvironments: an architectural platform for the convergence and integration of transcriptional regulatory signals. Eur J Histochem 48:65-76

Stein, Gary S; Lian, Jane B; van Wijnen, Andre J et al. (2004) Runx2 control of organization, assembly and activity of the regulatory machinery for skeletal gene expression. Oncogene 23:4315-29

Stein, Gary S; Lian, Jane B; Montecino, Martin et al. (2003) Nuclear microenvironments support physiological control of gene expression. Chromosome Res 11:527-36

Lian, Jane B; Stein, Janet L; Stein, Gary S et al. (2003) Runx2/Cbfa1 functions: diverse regulation of gene transcription by chromatin remodeling and co-regulatory protein interactions. Connect Tissue Res 44 Suppl 1:141-8

Stein, Gary S; Lian, Jane B; Stein, Janet L et al. (2003) Temporal and spatial parameters of skeletal gene expression: targeting RUNX factors and their coregulatory proteins to subnuclear domains. Connect Tissue Res 44 Suppl 1:149-53

Stein, Gary S; Lian, Jane B; Stein, Janet L et al. (2003) Intranuclear organization of RUNX transcriptional regulatory machinery in biological control of skeletogenesis and cancer. Blood Cells Mol Dis 30:170-6

Stein, Gary S; Lian, Jane B; Stein, Janet L et al. (2003) Intranuclear trafficking of transcription factors: Requirements for vitamin D-mediated biological control of gene expression. J Cell Biochem 88:340-55

Drissi, Hicham; Pouliot, Arlyssa; Koolloos, Christian et al. (2002) 1,25-(OH)2-vitamin D3 suppresses the bone-related Runx2/Cbfa1 gene promoter. Exp Cell Res 274:323-33

Zaidi, Sayyed K; Sullivan, Andrew J; van Wijnen, Andre J et al. (2002) Integration of Runx and Smad regulatory signals at transcriptionally active subnuclear sites. Proc Natl Acad Sci U S A 99:8048-53