Among the myosin super-family of motor proteins, myosin II is the sole filament forming class. Myosin IIs are hexamers containing 2 identical heavy chains and 2 pairs of light chains, the essential light chain (ELC) and the regulatory light chain (RLC) and a long coiled-coil dimerization domain, S2, that extends into the LMM filament forming domain. Myosin isoforms that are """"""""regulated"""""""" depend either on RLC phosphorylation, e.g., arthropod, smooth muscle and nonmuscle myosins, or Ca2+ binding, e.g., molluscan striated muscles. Myosin based regulation has in common a dependency on intramolecular interactions between the two myosin heads. This interaction was visualized in smooth muscle myosin and recently in arthropod thick filaments suggesting that a head-head interaction and a conformational change to a compact structure is a general feature of myosin II regulation. Biochemistry and modeling studies have identified a number of the features of the conformational change among which are torsional motions about the S2 alpha-helices that are dependent on the length of S2. These torsional motions could affect other myosin functions, including double headed interactions with the actin filament and processive motion. This grant application proposes to investigate structural and functional features of regulation in smooth muscle myosin that have a strong dependency on the length of the coiled-coil domain. Myosin V, a cytoplasmic myosin also forms a compact inhibited conformation with some similarity to that of myosin II, but with an interaction between the myosin heads and the cargo binding domain. Both myosin II and myosin V bind actin when in the inhibited conformation, but with very different affinities. While myosin V is widely identified as a processive motor, which requires simultaneous actin binding by both heads, simultaneous binding of smooth muscle myosin by both heads is controversial. The inhibited states of both myosin II and myosin V have common underlying principles. This project seeks answers to these and other aspects of two headed motor function and regulation. Finally, inhibited conformations in conventional kinesin also involve an interaction between the cargo binding domains and the kinesin heads. This conformation may have structural and functional similarities with inhibited conformations of myosin and will also be investigated.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR047421-10
Application #
8064021
Study Section
Macromolecular Structure and Function C Study Section (MSFC)
Program Officer
Boyce, Amanda T
Project Start
2001-02-15
Project End
2013-03-31
Budget Start
2011-04-01
Budget End
2013-03-31
Support Year
10
Fiscal Year
2011
Total Cost
$294,366
Indirect Cost
Name
Florida State University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
790877419
City
Tallahassee
State
FL
Country
United States
Zip Code
32306
Márquez, G; Pinto, A; Alamo, L et al. (2014) A method for 3D-reconstruction of a muscle thick filament using the tilt series images of a single filament electron tomogram. J Struct Biol 186:265-72
Taylor, Kenneth A; Feig, Michael; Brooks 3rd, Charles L et al. (2014) Role of the essential light chain in the activation of smooth muscle myosin by regulatory light chain phosphorylation. J Struct Biol 185:375-82
Winkler, Hanspeter; Wu, Shenping; Taylor, Kenneth A (2013) Electron tomography of paracrystalline 2D arrays. Methods Mol Biol 955:427-60
Baumann, Bruce A J; Taylor, Dianne W; Huang, Zhong et al. (2012) Phosphorylated smooth muscle heavy meromyosin shows an open conformation linked to activation. J Mol Biol 415:274-87
Winkler, Hanspeter; Zhu, Ping; Liu, Jun et al. (2009) Tomographic subvolume alignment and subvolume classification applied to myosin V and SIV envelope spikes. J Struct Biol 165:64-77
Taylor, Dianne W; Kelly, Deborah F; Cheng, Anchi et al. (2007) On the freezing and identification of lipid monolayer 2-D arrays for cryoelectron microscopy. J Struct Biol 160:305-12
Taylor, Kenneth A (2007) Regulation and recycling of myosin V. Curr Opin Cell Biol 19:67-74
Schoffstall, Brenda; Brunet, Nicolas M; Williams, Shanedah et al. (2006) Ca2+ sensitivity of regulated cardiac thin filament sliding does not depend on myosin isoform. J Physiol 577:935-44
Tama, Florence; Feig, Michael; Liu, Jun et al. (2005) The requirement for mechanical coupling between head and S2 domains in smooth muscle myosin ATPase regulation and its implications for dimeric motor function. J Mol Biol 345:837-54
Liu, Jun; Reedy, Mary C; Goldman, Yale E et al. (2004) Electron tomography of fast frozen, stretched rigor fibers reveals elastic distortions in the myosin crossbridges. J Struct Biol 147:268-82

Showing the most recent 10 out of 11 publications