Since its inception in 1965, the long term goals of this project have been to design, synthesize and evaluate new radiopharmaceuticals as potentials organ or tumor-imaging agents.
A specific aim has been to analyze various strategies for accomplishing the site-specific delivery of radiopharmaceuticals to specific tissues. Since these studies have focused on radioiodine as the imaging nuclide, a key factor in achieving the goal has been to select appropriate molecules to serve in the transport of radioiodine to the desired target. Accordingly, the design of the radioiodinated molecules has drawn heavily from current knowledge on the fate and disposition of xenobiotics(e.g. chloroquine-melanin affinity, bretylium-adrenergic neuron) and the synthesis and metabolism of biochemically-important molecules (e.g. cholesterol-adrenal cortex, phospholipid ethers-tumors.) Results with two classes, namely the radioiodinated phospholipid ethers (PLE) and radioiodinated triglyceride analogs, have shown sufficient promise that the current grant period will focus entirely on these agents. For example, several radioiodinated PLE have been shown to accumulate in a variety of tumors including the rat Walker 256 carcinosarcoma, the rabbit Vx2 adenocarcinoma, and human malignant melanoma (HTB63), small cell carcinoma (NCI-69), colon carcinoma(LS-180),and ovarian carcinoma (HTB77)carried in athymic mice. In most instances tumor to blood ratios exceeded 25 and the tumors could be readily imaged in vivo by gamma camera scintigraphy. In a similar manner, certain radioiodinated triglyceride analogs have been shown to be taken up by the liver in concentrations >50% of the administered dose and also to be substrates for both lipoprotein lipase and hepatic lipase such that they are excellent candidates for mapping global and regional hepatic lipid metabolism. Therefore, the goals of this project are (1) to undertake the necessary chemical, pharmacological and toxicological studies with appropriate radioiodinated PLE and triglycerides in order (2) to utilize such data to obtain approval from the Radioactive Drug Research Committee (RDRC) to perform preliminary biodistribution studies in normal volunteers and patients with documented metastatic neoplasms (in the case of PLE) and liver dysfunction (in the case of a triglyceride analog) as a prelude to submission of investigational new drug (I.N.D.) applications for expanded evaluation of clinical efficacy.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA008349-25
Application #
3163312
Study Section
Diagnostic Radiology Study Section (RNM)
Project Start
1979-05-01
Project End
1994-06-30
Budget Start
1992-07-01
Budget End
1993-06-30
Support Year
25
Fiscal Year
1992
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
Schools of Medicine
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
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Plotzke, K P; Rampy, M A; Meyer, K et al. (1993) Biodistribution, metabolism, and excretion of radioiodinated phospholipid ether analogs in tumor-bearing rats. J Nucl Biol Med 37:264-72
Schwendner, S W; Weichert, J P; Longino, M A et al. (1992) Potential organ or tumor imaging agents. 32. A triglyceride ester of p-iodophenyl pentadecanoic acid as a potential hepatic imaging agent. Int J Rad Appl Instrum B 19:639-50
Plotzke, K P; Haradahira, T; Stancato, L et al. (1992) Selective localization of radioiodinated alkylphosphocholine derivatives in tumors. Int J Rad Appl Instrum B 19:765-73
Deforge, L E; Degalan, M R; Ruyan, M K et al. (1992) Comparison of methods for incorporating a radioiodinated residualizing cholesteryl ester analog into low density lipoprotein. Int J Rad Appl Instrum B 19:775-82
DeForge, L E; Ruyan, M K; Schwendner, S W et al. (1991) Synthesis and evaluation of radioiodinated cholesteryl ethers as lipoprotein probes. Bioconjug Chem 2:254-60
Freeman, D A; Counsell, R E (1991) Cellular internalization, transport, and esterification of iodine-125-NP59 by MA-10 Leydig tumor cells. J Nucl Med 32:495-9
Counsell, R E; Schwendner, S W; DeForge, L E et al. (1991) Lipoproteins as carriers for organ-imaging radiopharmaceuticals. Targeted Diagn Ther 5:251-314
Counsell, R E; Schwendner, S W; Meyer, K L et al. (1990) Tumor visualization with a radioiodinated phospholipid ether. J Nucl Med 31:332-6
Strawn, L M; Martell, R E; Simpson, R U et al. (1989) Synthesis and evaluation of iodinated analogues of diacylglycerols as potential probes for protein kinase C. J Med Chem 32:643-8

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